Role of soil bacterial consortia on glyphosate degradation and growth of maize seedlings
Pre-growing weed control by glyphosate herbicides is effective for increasing yield, but glyphosate residues in the soil might reduce soil quality and can accumulate in agricultural products. Naturally, microbes are able to breakdown glyphosate into nontoxic substances orthophosphate and glycine. Glyphosate degradation in soil by single soil microbes are reported elsewhere, but the information about glyphosate removal by soil bacterial consortia was limited. The objective of this research was to determine the effect of carbon (C), nitrogen (N), and phosphorus (P) composition in liquid media to increase glyphosate degradation and its degradation product by soil bacterial consortia and 2) verify the effect of bacterial consortia on maize seedlings growth, their N and P uptake, as well as total and soluble P in soil. Glyphosate degradation test was set up by incubating bacterial consortia in a different composition of C-N-P liquid basal media. Greenhouse experiment has been performed in a randomized block design to treat maize grown in Inceptisols with bacterial and glyphosate application. The results showed that C-N-P composition of liquid media affected the concentration of glyphosate, as well as orthophosphate and glycine as by-products. In-planta experiment verified that inoculation of glyphosate-degrading bacterial to maize seedling grown in glyphosate-contaminated soil enabled to enhance shoot dry weight of maize seedling and N and P uptake at 4 weeks after inoculation.
Alexander, A., Singh, V.K., Mishra, A. and Jha, B. 2019. Plant growth promoting rhizobacterium Stenotrophomonas maltophilia BJ01 augments endurance against N2 starvation by modulating physiology and biochemical activities of Arachis hypogea. PLoS ONE 14(9): e0222405.
Alsop, G.M. Wage, G.T. and Coney, R.A. 1980. Bacterial growth inhibition test. Journal of the Water Pollution Control Federation 52(10): 2452-2456.
Arfarita, N., Imai, T. and Prasetya, B. 2014. Potential use of soil-born fungi isolated from treated soil in Indonesia to degrade glyphosate herbicide. Journal of Degraded and Mining Lands Management 1(2): 63-68.
Arfarita, N., Djuhari, D., Prasetya, B. and Imai, T. 2016. The application of Trichoderma viride strain FRP3 for biodegradation of glyphosate herbicide in contaminated land. AGRIVITA Journal of Agricultural Science 38(3):275-281.
Benslama, O. and Boulahrouf, A. 2013. Isolation and characterization of glyphosate-degrading bacteria from different soils of Algeria. African Journal of Microbiology Research 7(49): 5587-5595.
Bohn, T., Cuhra, M., Traavik, T., Sanden, M., Fagan, J. and Primicerio, R. 2014. Compositional differences in soybeans on the market: glyphosate accumulates in Roundup Ready GM Soybeans. Food Chemistry 153: 207 – 215.
Catrinck, T.C.P.G., Dias, A., Aquiar, M.C.S., Silverio, F.O., Fidencio, P.H. and Pinho, G.P. 2014. A simple and efficient method for derivatization of glyphosate and AMPA using 9-fluorenylmethyl chloroformate and spectrophotometric analysis. Journal of Brazillian Chemical Society 25(7):1194-1199.
Cattani, D., Cavalli, V.L.L.O., Heinz, R.C.E., Tonietto, D.J., Tharine, D-C., Ines, T.C., Barreto, S.F.R.M. and Ariane, Z. 2014. Mechanism underlying the neurotoxicity induced by glyphosate-based herbicide in immature rat hippocampus: involvement of glutamate excitotoxicity. Toxicology 320: 34-45.
Condrosari, P., Komarya, A., Suryatmana, P., Hariyadi, H.R. and Hindersah, R. 2018. Growth inhibition test of glyphosate herbicide for glyphosate-degrading-bacteria screening. International Journal of ChemTech Research 11(5): 240-248.
Cox, C. 1998. Glyphosate (Roundup). Journal of Pesticide Reform 18(3): 3–17.
Doran, P.M. 1995. Bioprocess Engineering Principles. Elsevier Science & Technology Books. 75p.
Duke, S.O. and Powles, S.B. 2008. Glyphosate: a once-in-a-century herbicide. Pest Management Science 64(4):319-325.
Duke, S.O., Lydon, J., Koskinen, W.C, Moorman, T.B., Chaney, R.L. and Hammerschmidt, R. 2012. Glyphosate effects on plant mineral nutrition, crop rhizosphere microbiota, and plant disease in glyphosate-resistant crops. Journal of Agricultural and Food Chemistry 60:10375-10397.
Faqihhudin, M.D., Haryadi, H. and Purnamawati, H. 2014. The use of glyphosate herbicides on growth, yield and residue of maize. Ilmu Pertanian 17(1):1 – 12 (in Indonesian).
Hashem, A., Tabassum, B. and Abd-Allah, E.F. 2019. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences 26(6): 1291-1297.
Hébert, M-P., Fugère, V. and Gonzalez, A. 2019. The overlooked impact of rising glyphosate use on phosphorus loading in agricultural watersheds. In Frontiere. Ecology and Environment 17(1):48-56.
Helander, M., Pauna, A., Saikkonen, K. and Saloniemi, I. 2019. Glyphosate residues in soil affect crop plant germination and growth. Scientific Reports, 9:no.19653.
Hove-Jensen, B., Zechel, D.L. and Jochimsen, B. 2014. Utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorus lyase. Microbiology and Molecular Biology Reviews 78(1):176 –197.
Iyer, R., Iken, B., Damania, A. and Krieger, J. 2018. Whole genome analysis of six organophosphate-degrading rhizobacteria reveals putative agrochemical degradation enzymes with broad substrate specificity. Environmental Science Pollution Research 25(14):13660-13675.
Kesuma, S.D., Hariyadi, H. and Anwar, S. 2015. The impact of IPA glyphosate herbicide application on a no-tillage system on rice and rice plant. Journal of Natural Resources and Environment 5(1):61-70 (in Indonesian).
Kryuchkova, Y.V., Burygin, G.L., Gogoleva, N.E., Gogolev, Y.V., Chernyshova, M.P., Makarov, O.E., Fedorov, E.E. and Turkovskaya, O.V. 2014. Isolation and characterization of a glyphosate-degrading rhizosphere strain Enterobacter cloacae K7. Microbiological Research 169(1): 99 - 105.
Landry, D., Dousset, S., Fournier, J-C and Andreux, F. 2005. Leaching of glyphosate and AMPA under two soil management practices in Burgundy vineyard (Vosne-Romanee, 21-France). Environmental Pollution 138(2):191-200.
Ma, Q., Cao, X.C., Xie, Y. Xiao, H., Tan, X. and Wu, L. 2017. Effects of glucose on the uptake and metabolism of glycine in pakchoi (Brassica chinensis L.) exposed to various nitrogen sources. Biomedical Central Plant Biology 17:no. 58.
Manogaran, M., Shukor, M.D., Yasid, N.A., Khalil, K.A. and Ahmad, S.A. 2018. Optimization of culture composition for glyphosate degradation by Burkholderia vietnamiensis strain AQ5-12. 3 Biotechnology 8(2): pp. 13.
Myers, J.P., Antoniou, M.N., Blumberg, B., Carroll, L., Colborn, T., Everett, Lg., Hansen, M., Landrigan, P.J., Lanphear, B.P., Mesnage, R., Vandenberg, L.N., Vom Saal, F.S., Welshons, W.V. and Benbrook, C.M. 2016. Concerns over use of glyphosate-based herbicides and risks associated with exposures: a consensus statement. Environmental Health 2(1):19.
Paytan, A. and Karen, M. 2007. Phosphorus in our waters. Oceanography 20(2): 200-206.
Rodríguez, M.P., Melo, C., Jiménez, E. and Dussán, A. 2019. Glyphosate bioremediation through the sarcosine oxidase pathway mediated by Lysinibacillus sphaericus in soils cultivated with potatoes. Agriculture 9(10): 217.
Sanders, E.R. 2012. Aseptic laboratory techniques: plating methods. Journal of Visualized Experiment 63:e3064.
Shah, S.A., Rathod, I.S. and Dharitri, K. 2007. Colorimetry method for estimation of glycine, alanine and isoleucine. Indian Journal of Pharmaceutical Sciences 69(3): 462-464.
Shehata, A.A., Schrödl, W., Aldin, A.A., Hafez, H.N. and Krüger, M. 2013. The effect of glyphosate on potential pathogens and beneficial members of poultry microbiota in vitro. Current Microbiology 66:350–358.
Song, C., Zhang, Y., Xia, X., Qi, H., Li, M., Pan, H. and Xi, B. 2018. Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste. Microbial Technology 11(6):1124-1136.
Vivancos, P.D., Driscoll, S.P., Bulman, C.A., Ying, L., Emami, K., Treumann, A., Mauve, C., Noctor, G. and Foyer, C.H. 2011. Perturbations of amino acid metabolism associated with glyphosate-dependent inhibition of shikimic acid metabolism affect cellular redox homeostasis and alter the abundance of proteins involved in photosynthesis and photorespiration. Plant Physiology 157(1): 256–268.
Yu, X.M., Yu, T., Yin, G.H., Dong, Q.L., An, M., Wang, H.R. and Ai, X.X. 2015. glyphosate biodegradation and potential soil bioremediation by Bacillus Subtilis strain BS-15. Genetic and Molecular Research 14(4):4717-30.
- There are currently no refbacks.
Copyright (c) 2020 Journal of Degraded and Mining Lands Management
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.