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Reginawanti Hindersah
Universitas Padjadjaran

Department of soil science

Zellya Handyman
Soil Science Department, Universitas Padjadjaran, Indonesia

Febby Nur Indriani


Pujawati Suryatmana


Nenny Nurlaeny


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Azotobacter population, soil nitrogen and groundnut growth in mercury-contaminated tailing inoculated with Azotobacter

Reginawanti Hindersah, Zellya Handyman, Febby Nur Indriani, Pujawati Suryatmana, Nenny Nurlaeny
  J. Degrade. Min. Land Manage. , pp. 1269-1274  
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Gold mine tailing in Buru island, Maluku Province, Indonesia contains high level of mercury but low in carbon and plant nutrients. Revegetation in such condition needs certain soil treatment which is suitable for plant growth. The objective of pot trials was to study the effect of indigenous Plant Growth Promoting Rhizobacteria Azotobacter and organic matter on bacterial survival as well as growth of groundnut grown in mine tailing. The experimental design was a Split Plot Design which tested three types of Azotobacter liquid inoculant and three soil total organic carbon (TOC) contents. Results showed that Azotobacter inoculation increased Azotobacter population in tailing at the end of vegetative growth of groundnut. Total nitrogen content in soil decreased when TOC level increased. However, nitrogen uptake and growth of groundnut did not change after Azotobacter inoculation or manure amendment. These experiments provided information that Azotobacter inoculation on groundnut maintain its proliferation in Hg-contaminated mine tailing.


Azotobacter, mercury-contaminated tailing, PGPR, revegetation

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Akratos, C.S., Tekerlekopoulou, A.G., Vasiliadou, I.A. and Vayenas, A.D. 2017. Cocomposting of olive mill waste combining aerobic biological treatment, constructed wetland and composting. In Galanakis, C.M. (ed), Olive Mill Waste Recent Advances for Sustainable Management, Elsevier Academic Press, London., pp 161-182.

Bell, J., Dunford, A.J.,E. Hollis, E. and Henderson, R.A. 2003. The role of Mo atoms in nitrogen fixation: balancing substrate reduction and dihydrogen production. Chemical International 42(10):1149–1152.

Bengtson, P. 2004. Microbial mobilization and immobilization of soil nitrogen. Dissertation, Lund University, Lund.

Carpa, R., Dobrotă, C., Keul-Butiucm, A., Maior, M.C., Muntean, V. and Drăgan-Bularda, M. 2010. Influence of pH on growth and nitrogen fixation in bacterial strains isolated from altitudinal vegetation zones of Parâng Mountains, Romania. Analele Universităţii din Oradea 17(1):76-83.

Downie, J.A. 2014. Legume nodulation. Current Biology 24(5): R184-R190.

Hindersah, R., Arief, D.H., Soemitro, S. and Gunarto, L. 2006. Exopolysaccharide Extraction from Rhizobacteria Azotobacter sp. Proceedings of the 5th Regional IMT-GT Uninet Conference & International Seminar.22-23 June 2006. Tiara Hotel, Medan, Indonesia. p 50-55.

Hindersah, R., Mulyani, O. and Osok, R. 2017. Proliferation and exopolysaccharide production of Azotobacter in the presence of mercury. Biodiversity 8 (1):21-26.

Hindersah, R., Risamasu, R., Kalay, A.M., Dewi, T. and Makatita, I. 2018. Mercury contamination in soil, tailing and plants on agricultural fields near closed gold mine in Buru Island, Maluku. Journal of Degraded and Mining Lands Management 5(2):1027-1034.

Hinsinger, P., Plassard, C. and Jaillard, B. 2006. Rhizosphere: A new frontier for soil biogeochemistry. ChemInform 88(1):210-213.

Huang, L., Baumgart, T. and Mulligan, D. 2012. Is rhizosphere remediation sufficient for sustainable revegetation of mine tailings?. Annual Botany 100: 223–238.

Janecka, J., Jenkins, M.B., Brackett, N.S., Lion, L.W. and Ghiorse, W.C. 2002. Characterization of a Sinorhizobium isolate and its extracellular polymer implicated in pollutant transport in soil. Applied and Environmental Microbiology 68(1):423–426.

Jadhav, S.U., Jadhav, U.U., Dawkar, V.V. and Govindwar, S.P. 2008. Biodegradation of disperse dye brown 3REL by microbial consortium of Galactomyces geotrichum TCC 1360 and Bacillus sp. VUS. Biotechnology and Bioprocess Engineering 13(2): 232-239.

Jimenez, D.J., Montaña, J.S. and Martinez, M.M. 2011. Characterization of free nitrogen fixing bacteria of the genus Azotobacter in organic vegetable-grown Colombian soils. Brazilian Journal of Microbiology 42(3):846–858. doi: 10.1590/ S1517-83822011000300003.

Koziel, M., Gebala, B. and Martyniuk, S. 2013. Response of soybean to seed inoculation with Bradyrhizobium japonicum and with mixed inoculants of B. japonicum and Azotobacter chroococcum. Polish Journal of Microbiology 62(4):457-460.

Kujawa, C. 2011. Cycloning of Tailing for the Production of Sand as TSF Construction Material. Proceedings of the 15th International Conference on Tailings and Mine Waste.6-9 November 2011, Vancouver, BC. p 1-11.

Onwurah, I.N.E. 1999. Lipid peroxidation and protein oxidation in Azotobacter vinelandii exposed to mercury, silver, crude oil, and fenton reagent. Journal of Toxicology Substance Mechanisms 18 (4): 167-176.

Patil, V. 2011. Production of indole acetic acid by Azotobacter sp. Recent Research in Science and Technology 3(12):14-16

Pepper, I.L., Zerzghi, H.G., Bengson, S.A., Iker, B.C., Banerjee, M.J. and Brooks, J.P. 2012. Bacterial populations within copper mine tailings: long-term effects of amendment with Class Abiosolids. Journal of Applied Microbiology 113 (3): 569 -577

Seefeldt, L.C., Hoffman, B.M. and Dean, D.R. 2009. Mechanism of Mo-dependent nitrogenase. Annual Review of Biochemistry 78: 701-722.

Shen, Z-J., Wang, Y-P., Sunan, Q.Y. and Wang, W. 2014. Effect of vegetation succession on organic carbon, carbon of humus acids and dissolved organic carbon in soils of copper mine tailings sites. Pedosphere 24(2): 271-279.

Steinnes, A. 2004. Mercury. In Alloway, B.J. (ed), Heavy Metal in Soils, Chapman & Hall, Glasgow, pp 411-428.

Sulieman, S. and Tran, LS.P. 2014. Symbiotic nitrogen fixation in legume nodules:metabolism and regulatory mechanism. International Journal of Molecular Science 15:19389-19393; doi:10.3390/ ijms151119389.

Swaroop, G., Bulbule, K.A., Parthasarathy, P., Shivakumar, Y., Muniswamy, R., Annamati R. and Priyanka, D. 2013. Application of Gold Ore Tailings (GOT) as a source of micronutrients for the growth of plants. International Journal of Scientific World 1(3): 68-78.

Takatsuka, H. and Umeda, M. 2014. Hormonal control of cell division and elongation along differentiation trajectories in roots. Journal of Experimental Botany 65(10): 2633–2643.

Taiz, L. and Zeiger, E. 2003. Plant Physiology, Sinauer Associates, Inc., Oxford, UK. 690p.

Tilak, K.V.B.R., Ranganayaki, N., Pal, K.K., De, R., Saxena, A.K., Nautiyal, C.S., Mittal, S., Tripathi, A.K. and Johri, B.N. 2005. Diversity of plant growth and soil health supporting bacteria. Current Science 89(1):136-150.

Tilak, K.V.B.R., Ranganayaki, N. and Manoharachar, C. 2006. Synergistic effects of plant-growth promoting rhizobacteria and Rhizobium on nodulation and nitrogen fixation by pigeon pea (Cajanus cajan). European Journal of Soil Science 57(1): 67-71.

Vikhe, P.S. 2014. Azotobacter species as a natural plant hormone synthesizer. Research Journal on Recent Science3 (IVC):59-63.

Weisany, W., Raei, Y. and Sllahverdipoor, K.H. 2013. Role of some of mineral nutrients in biological nitrogen fixation. Bulletin of Environment Pharmacy and Life Science 2(4): 77-84.


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