The potential use of rhizobacteria and cover crop residue for improving the growth of sorghum on post-nickel mining soil in Southeast Sulawesi
DOI:
https://doi.org/10.15243/jdmlm.2025.122.7277Keywords:
biomass accumulation, bioremediation, growth performance, soil improvement, Sorghum sp.Abstract
The utilization of rhizobacteria and cover crop residue for improving plant productivity has been widely applied in agricultural land. However, the effectiveness of those treatments for increasing the growth performance of crops in the post-mining soil is still not evaluated. This study investigated the potential use of rhizobacteria and cover crop residue for improving the growth of Sorghum sp. developed in the post-mining soil in Southeast Sulawesi. An experiment was established at the laboratory level using a completely randomized design with three replicates for every treatment. Four treatments were examined in this trial comprising of CE (control+Eleusine indica), CC (control+Centrosema sp.), BE (Bacillus sp.+Eleusine indica), and BC (Bacillus sp.+Centrosema sp.). Some parameters were observed to evaluate the growth of Sorghum sp., namely diameter, height, total leaves, leaf area, and biomass. The results demonstrated the use of rhizobacteria and cover crop residue potentially improves the growth of Sorghum sp. in the post-mining soil even though there was not a significant difference among treatments statistically. The highest average biomass of Sorghum sp. was noted in BE (3.72±1.06 g), BC (3.20±0.50 g), CC (2.95±1.40 g), CE (2.81±0.33 g). These findings indicated that the treatment of BE was the best way to improve the growth performance of Sorghum sp. at the post-mining. According to the results, this study concluded that rhizobacteria and cover crops play a key role in soil fertility. The utilization of rhizobacteria and cover crop residue has the potential to support the growth of Sorghum sp. developed in the post-nickel mining soil.
References
Adetunji, A.T., Ncube, B., Mulidzi, R. and Lewu, F.B. 2020. Management impact and benefit of cover crops on soil quality: A review. Soil and Tillage Research 204:104717. https://doi.org/10.1016/j.still.2020.104717
Andrade, L.A.. De, Santos, C.H.B., Frezarin, E.T., Sales, L.R. and Rigobelo, E.C. 2023. Plant growth-promoting rhizobacteria for sustainable agricultural production. Microorganisms 11:1088. https://doi.org/10.3390/microorganisms11041088
Asari, S., Tarkowska, D., Rolcik, J., Novak, O., Palmero, D.V., Bejai, S. and Meijer, J. 2017. Analysis of plant growth-promoting properties of Bacillus amyloliquefaciens UCMB5113 using Arabidopsis thaliana as host plant. Planta 245(1):15-30. https://doi.org/10.1007/s00425-016-2580-9
Backer, R., Rokem, J.S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S. and Smith, D.L. 2018. Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science 871:1-17. https://doi.org/10.3389/fpls.2018.01473
Beyene, K. 2016. Assessing univariate and multivariate homogeneity of variance: A guide for practitioners. Journal of Mathematical Theory and Modeling 6:13-17.
Egli, M., Sartori, G., Mirabella, A., Giaccai, D., Favilli, F., Scherrer, D., Krebs, R. and Delbos, E. 2010. The influence of weathering and organic matter on heavy metals lability in silicatic, Alpine soils. Science of the Total Environment 408(4):931-946. https://doi.org/10.1016/j.scitotenv.2009.10.005
Estefan, G., Sommer, R. and Ryan, J. 2013. Methods of Soil, Plant, and Water Analysis: A Manual for the West Asia and North Africa Region. Third Edition, International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, 84-105.
Fernandez-Caliani, J.C., Giraldez, M.I., Waken, W., Del Rio, Z.M. and Cordoba, F. 2021. Soil quality changes in an Iberian pyrite mine site 15 years after land reclamation. Catena 206:105538. https://doi.org/10.1016/j.catena.2021.105538
Ghasemi, A. and Zahediasl, S. 2012. Normality tests for statistical analysis: A guide for non-statisticians. International Journal of Endocrinology and Metabolism 10(2):486-489. https://doi.org/10.5812/ijem.3505
Gouda, S., Kerry, R.G., Das, G., Paramithiotis, S., Shin, H.S. and Patra, J.K. 2018. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research 206:131-140. https://doi.org/10.1016/j.micres.2017.08.016
Grzyb, A., Wolna-Maruwka, A. and Niewiadomska, A. 2020. Environmental factors affecting the mineralization of crop residues. Agronomy 10(12):1-18. https://doi.org/10.3390/agronomy10121951
Hamzah, A., Hapsari, I.R. and Priyadarshini, R. 2017. The potential of wild vegetation species of Eleusine indica L., and Sonchus arvensis L. for phytoremediation of Cd-contaminated soil. Journal of Degraded and Mining Lands Management 4(3):797-805. https://doi.org/10.15243/jdmlm.2017.043.797
Hasan, A., Tabassum, B., Hashim, M. and Khan, N. 2024. Role of plant growth promoting rhizobacteria (PGPR) as a plant growth enhancer for sustainable agriculture: A review. Bacteria 2024(3):59-75. https://doi.org/10.3390/bacteria3020005
Igiehon, B.C., Babalola, O.O. and Hassen, A.I. 2024. Rhizosphere competence and applications of plant growth-promoting rhizobacteria in food production A review. Scientific African 23:e02081. https://doi.org/10.1016/j.sciaf.2024.e02081
Indriani, I., Hasan, S., Nompo, S., Ako, A., Rusdi, M. and Purnama, B.N. 2019. Introduction of Centro legume (Centrosema pubescens) and dwarf Napier grass (Pennisetum purpureum cv. Mott) in improving production and quality of grassland. International Journal of Scientific and Research Publications 9(3):234-239. https://doi.org/10.29322/IJSRP.9.03.2019.p8737
Iqbal, Z., Ahmad, M., Ali, M., Hilger, T. and Rasche, F. 2024. Phosphate?solubilizing Bacillus sp. modulate soil exoenzyme activities and improves wheat growth. Microbial Ecology 87:31. https://doi.org/10.1007/s00248-023-02340-5
John, R.C., Itah, A.Y., Essien, J.P. and Ikpe, D.I. 2011. Fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol. Bulletin of Environmental Contamination and Toxicology 87(3):343-353. https://doi.org/10.1007/s00128-011-0320-1
Karimuna, S.R., Wahab, A., Warda, and Baharudin. 2020. The effectiveness of fertilizing to increase growth and productivity of sorghum on dry land and marginal in Southeast Sulawesi. IOP Conference Series: Earth and Environmental Science 484:1-10. https://doi.org/10.1088/1755-1315/484/1/012073
Kashyap, B.K., Solanki, M.K. and Pandey, A.K. 2019. Bacillus as plant growth promoting rhizobacteria (PGPR): A promising green agriculture technology. In: Plant Health Under Biotic Stress (pp. 219-236). Springer Nature Singapore. https://doi.org/10.1007/978-981-13-6040-4
Kocira, A., Staniak, M., Tomaszewska, M., Kornas, R., Cymerman, J., Panasiewicz, K. and Lipinska, H. 2020. Legume cover crops as one of the elements of strategic weed management and soil quality improvement. A review. Agriculture 10. https://doi.org/10.3390/agriculture10090394
Koudahe, K., Allen, S.C. and Djaman, K. 2022. Critical review of the impact of cover crops on soil properties. International Soil and Water Conservation Research 10:343-354. https://doi.org/10.1016/j.iswcr.2022.03.003
Kumar, M., Giri, V.P., Pandey, S., Gupta, A. and Patel, M.K. 2021. Plant-growth-promoting rhizobacteria emerging as an effective bioinoculant to improve the growth, production, and stress tolerance of vegetable crops. International Journal of Molecular Sciences 22:12245. https://doi.org/10.3390/ijms222212245
Leomo, S., Alam, S., Afrianto, E., Jamil, L.O. and Muhidin. 2021. Cover crop residue effects on soil and corn performance in ex-nickel mining soils. Pakistan Journal of Biological Sciences 24(8):888-894. https://doi.org/10.3923/pjbs.2021.888.894
Leomo, S., Tufaila, M., Adawiyah, R., Rakian, T.C., Muhidin, Alam, S., Mudi, L. and Sutariati, G.A.K. 2019. The effectiveness of cover crop and rhizobacteria as amelioration on ex-nickel mine soil southeast Sulawesi. IOP Conference Series: Earth and Environmental Science 260:012146. https://doi.org/10.1088/1755-1315/260/1/012146
Miljakovic, D., Marinkovic, J. and Balesevic-Tubic, S. 2020. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms 2020, 8(7):1-19. https://doi.org/10.3390/microorganisms8071037
Mishra, P., Pandey, C.M. and Singh, U. 2019. Descriptive statistics and normality tests for statistical data. Annals of Cardiac Anaesthesia 22(1):67-72. https://doi.org/10.4103/aca.ACA_157_18
Muddarisna, N. and Siahaan, B.C. 2014. Application of organic matter to enhance phytoremediation of mercury-contaminated soils using local plant species: a case study on small-scale gold mining locations in Banyuwangi of East Java. Journal of Degraded and Mining Lands Management 2(1):251-258. https://doi.org/10.15243/jdmlm.2014.021.251
Mustikaningrum, D., Suprayogo, D. and Utami, S.R. 2018. Conservation farming in rain-fed agriculture: can biogeotextile, cover crop residues, and soil tillage application improve the growth and the yield of maize (Zea mays L.)? Journal of Degraded and Mining Lands Management 6(1):1409-1417. https://doi.org/10.15243/jdmlm.2018.061.1409
Nugroho, Y., Rudy, G.S., Hardiyanto, Y., Saputra, E., Alam, S., Matatula, J. and Wirabuana, P.Y.A.P. 2022. A comparison of soil characteristics from four land covers around a coal mining concession area in South Kalimantan. Journal of Degraded and Mining Lands Management 10(1):3883-3888. https://doi.org/10.15243/jdmlm.2022.101.3883
Paramasivam, C.R. and Anbazhagan, S. 2019. Soil fertility analysis in and around magnesite mines, Salem, India. Geology, Ecology, and Landscapes 4(2):140-150. https://doi.org/10.1080/24749508.2019.1608407
Pipai, R., Mcneill, A., Unkovich, M., Banabas, M. and Nelson, P.N. 2023. Biological nitrogen fixation by legume cover plants in oil palm plantations: calibration of the ureide technique and effects of plantation age and soil nitrate. Plant and Soil 491(1):665-680. https://doi.org/10.1007/s11104-023-06147-8
Pratiwi, Narendra, B.H., Siregar, C.A., Turjaman, M., Hidayat, A., Rachmat, H.H., Mulyanto, B., Suwardi, Iskandar, Maharani, R., Rayadin, Y., Prayudyaningsih, R., Yuwati, T.W., Prematuri, R. and Susilowati, A. 2021. Managing and reforesting degraded post-mining landscape in Indonesia: A review. Land 10(6):658. https://doi.org/10.3390/land10060658
Purwanto, B.H. and Alam, S. 2020. Impact of intensive agricultural management on carbon and nitrogen dynamics in the humid tropics. Soil Science and Plant Nutrition 66(1):50-59. https://doi.org/10.1080/00380768.2019.1705182
Quintarelli, V., Radicetti, E., Allevato, E., Stazi, S.R., Haider, G., Abideen, Z., Bibi, S., Jamal, A. and Mancinelli, R. 2022. Cover crops for sustainable cropping systems: A review. Agriculture 2022(12):2076. https://doi.org/10.3390/agriculture12122076
Rashid, A., Schutte, B.J., Ulery, A., Deyholos, M.K., Sanogo, S., Lehnhoff, E.A. and Beck, L. 2023. Heavy metal contamination in agricultural soil: environmental pollutants affecting crop health. Agronomy 13(6):1521. https://doi.org/10.3390/agronomy13061521
Sadono, R., Wardhana, W., Wirabuana, P.Y.A.P. and Idris. 2021. Soil chemical properties influences on the growth performance of Eucalyptus urophylla planted in dryland ecosystems, East Nusa Tenggara. Journal of Degraded and Mining Lands Management 8(2):2635-2642. https://doi.org/10.15243/jdmlm.2021.082.2635
Saeed, Q., Xiukang, W., Haider, F.U., Mumtaz, M.Z., Holatko, J., Naseem, M., Kintl, A., Ejaz, M., Naveed, M., Brtnicky, M. and Mustafa, A. 2021. Rhizosphere bacteria in plant growth promotion, biocontrol, and bioremediation of contaminated sites: A comprehensive review of effects and mechanisms. International Journal of Molecular Sciences 22:10529. https://doi.org/10.3390/ijms221910529
Sanchez-Castro, I., Molina, L., Prieto-Ferandez, M. and Segura, A. 2023. Past, present and future trends in the remediation of heavy-metal contaminated soil- remediation techniques applied in real soil-contamination events. Heliyon 9:e16692. https://doi.org/10.1016/j.heliyon.2023.e16692
Santos, R., Andrea, P., Diaz, E., Lana, L. and Lobo, B. 2020. Use of plant growth-promoting rhizobacteria in maize and sugarcane: Characteristics and applications. Frontiers in Food System 4:1-15. https://doi.org/10.3389/fsufs.2020.00136
Saxena, A.K., Kumar, M., Chakdar, H., Anuroopa, N. and Bagyaraj, D.J. 2020. Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology 128(6):1583-1594. https://doi.org/10.1111/jam.14506
Scavo, A., Fontanazza, S., Restuccia, A., Pesce, G.R., Abbate, C. and Mauromicale, G. 2022. The role of cover crops in improving soil fertility and plant nutritional status in temperate climates. A review. Agronomy for Sustainable Development Volume 42, Article number 93. https://doi.org/10.1007/s13593-022-00825-0
Solis, R., Pezo, M., Arevalo, L., Lao, C., Alegre, J. and Perez, K. 2019. Evaluation of leguminous species as cover crops associated with sacha inchi. Pesquisa Agropecuaria Tropical 49:e58011. https://doi.org/10.1590/1983-40632019v4958011
Stepien, A., Wojtkowiak, K. and Kolankowska, E. 2022. Effect of commercial microbial preparations containing Paenibacillus azotofixans, Bacillus megaterium and Bacillus subtilis on the yield and photosynthesis of winter wheat and the nitrogen and phosphorus content in the soil. Applied Sciences 12:12541. https://doi.org/10.3390/app122412541
Syaf, H., Pattah, M.A. and Kilowasid, L.M.H. 2022. Quality of soil from the nickel mining area of Southeast Sulawesi, Indonesia, engineered using earthworms (Pheretima sp.). Journal of Degraded and Mining Lands Management 8(4):2295-3005. https://doi.org/10.15243/jdmlm.2021.084.2995
Teitzel, J.K. and Burt, R.L. 1976. Centrosema pubescens in Australia. Tropical Grasslands 10:5-14.
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S. and Nasrulhaq, B.A. 2016. Role of plant growth promoting rhizobacteria in agricultural sustainability: A review. Molecules 21(5):1-17. https://doi.org/10.3390/molecules21050573
Widawati, S. and Suliasih, S. 2019. Role of indigenous nitrogen-fixing bacteria in promoting plant growth on post tin mining soil. Makara Journal of Science 23(1):28-38. https://doi.org/10.7454/mss.v23i1.10801
Widdig, M., Schleuss, P.M., Weig, A.R., Guhr, A., Biederman, L.A., Borer, E.T., Crawley, M.J., Kirkman, K.P., Seabloom, E.W., Wragg, P.D. and Spohn, M. 2019. Nitrogen and phosphorus additions alter the abundance of phosphorus-solubilizing bacteria and phosphatase activity in grassland soils. Frontiers in Environmental Science 7:15. https://doi.org/10.3389/fenvs.2019.00185
Wirabuana, P.Y.A.P., Alam, S., Matatula, J., Harahap, M.M., Nugroho, Y., Idris, F., Meinata, A. and Sekar, D.A. 2021. The growth, aboveground biomass, crown development, and leaf characteristics of three eucalyptus species at the initial stage of planting in Jepara, Indonesia. Biodiversitas 22(5):2859-2869. https://doi.org/10.13057/biodiv/d220550
Yang, S.X., Liao, B., Li, J.T., Guo, T. and Shu, W.S. 2010. Acidification, heavy metal mobility and nutrient accumulation in the soil-plant system of a revegetated acid mine wasteland. Chemosphere 80(8):852-859. https://doi.org/10.1016/j.chemosphere.2010.05.055
Zhakypbek, Y., Kossalbayev, B.D., Belkozhayev, A.M., Murat, T., Tursbekov, S., Abdalimov, E., Pashkovskiy, P., Kreslavski, V., Kuznetsov, V. and Allakhverdiev, S.I. 2024. Reducing heavy metal contamination in soil and water. Plants 2024, 13(11):1534. https://doi.org/10.3390/plants13111534
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