Effect of gypsum and cow manure on yield, proline content, and K/Na ratio of soybean genotypes under saline conditions


  • Wiwin Sumiya Dwi Yamika Universitas Brawijaya http://orcid.org/0000-0001-5720-4248
  • Nurul Aini Universitas Brawijaya
  • Adi Setiawan Universitas Brawijaya
  • Runik Dyah Purwaningrahayu Indonesian Legumes and Tuber Crops Research Institute, Indonesia




ameliorant, chlorophyll, K/Na ratio, proline, salinity, soybean


Gypsum and cow manure potential as ameliorant to increase crop production under salt stress or saline condition. This research aimed to learn the effect of gypsum and cow manure on the uptake of Na, K and the yield of soybean genotypes under saline condition. This research conducted in green house Jatikerto Experimental Farm Faculty of Agriculture, Brawijaya University, from June to September 2014. The research was arranged in a split plot design. The main plot was soybean genotypes consists of two saline susceptible varieties (G1 = Wilis and G2 = Tanggamus) and two saline tolerant genotypes (G3 = genotype IAC, 100/Bur//Malabar and G4 = genotype Argopuro//IAC, 100); sub plot was ameliorant application consists of A0 = without ameliorant; A1 = cow manure (20 t/ha); and A2 = gypsum (5 t/ha). The results of the research showed that Leaf Chlorophyll Index in susceptible varieties and tolerant genotypes were increased with ameliorant application. Accumulation of proline and K/Na ratio in susceptible varieties higher than tolerant genotypes. Ameliorant application on tolerant genotypes increased grain yield higher than susceptible varieties

Author Biographies

Wiwin Sumiya Dwi Yamika, Universitas Brawijaya

Departement of Agronomy

Nurul Aini, Universitas Brawijaya

Departement Agronomy

Adi Setiawan, Universitas Brawijaya

Departement Agronomy

Runik Dyah Purwaningrahayu, Indonesian Legumes and Tuber Crops Research Institute, Indonesia



Aini, N., Mapfumo, E., Rengel, Z. and Tang, C. 2012. Ecophysiological responses of Melaleuca species to dual stresses of water logging and salinity. International. Journal of Plant Physiology and Biochemistry 4 (4): 52 – 58.

Aini, N., Yamika, W.S.D., Purwaringrahayu, R.D. and Setiawan, A. 2014. Growth And Physiological Characteristics Of Soybean Genotypes (Glycine max L.) Toward Salinity Stress. Agrivita 36 (3) : 201 – 209.

Akgun, I., Kara, B. and Altindal, D. 2011. Effect of salinity (NaCl) on germination, seedling growth and nutrient uptake of different triticale genotypes. Turkish Journal of Field Crops 16(2): 225-232.

Alikhani, F., Saboora, A. and Razavi, K. 2011. Changes in osmolites contents, lipid peroxidation and photosynthetic pigment of Aeluropus lagopoides under potassium deficiency and salinity. Journal of Stress Physiology & Biochemistry 7( 2): 5-19.

Asch, F., Dingkuhn, M., DÓ§rffling, K. and Miezan, K. 2000. Leaf K/Na ratio predicts salinity induced yield loss in irrigated rice. Euphytica 113: 109-118.

Bates, L.S., Waldron, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil 39: 205-208.

Biro Pusat Stastistik (BPS). 2013. production of Rice, Corn and Soybeans. No 73/11/Th. XVI, 1 November 2013. http://www.bps.go.id/brs_file/aram_01nov13. .(Accessed July 12, 2014)

Bot, A. and Benites, J. 2005. The importance of soil organic matter key to drought resistant soil and sustained food production. Food And Agriculture Organization of The United Nations. Rome.

Cha-um, S, and Kirdmanee, C. 2009. Effect of salt stress on proline accumukation photosynthetic ability and growth character in two maize cultivars. Pakistan Journal of Botany 4(1): 87 – 98.

Clark, R.B., and Baligar, V.C. 2003. Growth of forages legumes and grasses in acidic soil amended with flue gas desulfurization products. Communications in Soil Science and Plant Analysis 34 (1 & 2): 157-180.

Frazen, D., Rehm, G. and Gerwing, J. 2006. Effectiveness of gypsum in the nort central region of the US. North Dakota University.

Golezani, K.G. and Noori, M.T. 2011. Soybean Performance under Salinity Stress, Soybean - Biochemistry, Chemistry and Physiology, Prof. Tzi-Bun Ng (Ed.), InTech, DOI: 10.5772/14741. Available from: https://www.intechopen.com/books/soybean-biochemistry-chemistry-and-physiology/soybean-performance-under-salinity-stress

Hanafiah, K.A. 2007. Fundamental of Soil Science. PT. Raja Grafindo Persada. Jakarta (in Indonesian).

In Tech. http://www.intechopen.com/books/soybean-biochemistry-chemistry-andphysiology/soybean-performance-under-salinity-stress. (Accessed July 20, 2015)

Jones, J.B. Jr. 2002. Agronomic handbook. Management of crops, soils and their fertility. CRC. Press. USA. pp.450

Li-ping, L, Xiao-hua,L., Hong-bo, S., Zhao-Pu, L., Ya, T., Quan-suo, Z. and Jun-qin, Z. 2015. Ameliorants improve saline-alkaline soils on a large scale in northern Jiangsu Province, China. Ecological Enginering 81 : 328 – 334.

Mahmoodabadi, M., Yazdanpanah, N., Sinobas, L.R., Pazira, E. and Neshat, A. 2012. Reclamation of calcareous saline sodic soil with different amendment (I) : redistribution of soluble cations within the soil profile. Agriculture Water Management http://dx.doi.org/10.1016/j.agwat.2012.08.018.

Nazarbeygi, E., Yazdi, H.L., Nazeri, R. and Soleimani, R. 2011. The effect of different levels of salinity on proline and a,b chlorophylls in canola. American Eurasian Journal Agriculture and Environment Science 10 (1): 70 -74.

Njoku, K.L., Akinola, M.O. and Oboh, B.O. 2008. Growth and performance of Glycine max L. (Merrill) grown in crude oil contaminated soil augmented with cow dung. Life Science Journal 5 (3): 89–93.

Patel, D. and Meenu S. 2012. Influence of soil ameliorants and microflora on induction of antioxidant enzymes and growth promotion of Jatropha curcas L. under saline condition. European Journal of Soil Biology 55 : 47 – 54.

Rachman, A., Subiksa, and Wahyunto. 2007. Expansion of soybean plants to sub-optimal land. Puslitbangtan, Bogor. p. 185-226.

Rady, M.M. 2012. A novel organo-mineral fertilizer can mitigate salinity stress effects for tomato production on reclaimed saline soil. South African Journal of Botany 81: 8–14.

Roesmarkam, A. and Yuwono, N.W. 2002. Soil Fertility. Kanisius. Yogyakarta. pp. 224 (in Indonesian)

Sasongko, P.E. and Warsito. 2003. Na salt behavior on some saline soil column height and application of soil amendment. Jurnal Penelitan Ilmu-ilmu Pertanian 3 (1) : 51-55 (in Indonesian)

Shafi, M., Bakht, J., Khan, M.J., Khan, M.A. and Raziuddin, A. 2011. Role of abscisic acid and proline in salinity tolerance of wheat genotypes. Pakistan Journal of Botany 43 (2): 1111 – 1118.

Simanungkalit, R.D.M., Suriadikarta, D.A., Saraswati, R., Setyorini, D. and Hartatik, W. 2006. Organic Fertilizer and Biofertilizer. Indonesian Center for Agricultural Land Resources Research and Development (ICALRRD).Bogor (in Indonesian).

Tejada, M., Garcia, C., Gonzalez,J.L. and Hernandez, M.T. 2006. Use of organic amendment as a strategy for saline soil remediation : influence on the physical, chemical and biological properties of soil. Soil Biology and Biochemistry 38 : 1413-1421.








How to Cite

Yamika, W. S. D., Aini, N., Setiawan, A., & Purwaningrahayu, R. D. (2018). Effect of gypsum and cow manure on yield, proline content, and K/Na ratio of soybean genotypes under saline conditions. Journal of Degraded and Mining Lands Management, 5(2), 1047–1053. https://doi.org/10.15243/jdmlm.2018.052.1047



Research Article

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