Utilization of biochar and Trichoderma harzianum to promote growth of shallot and remediate lead-contaminated soil

Authors

DOI:

https://doi.org/10.15243/jdmlm.2021.083.2743

Keywords:

biochar, lead, remediation, shallot, T.harzianum

Abstract

This study aimed to determine the effect of biochar and Trichoderma harzianum toward lead removal in soil, lead absorption, lead content in plant tissue also growth and yield of shallot cultivated on lead-contaminated soil. The experimental design used was a completely factorial randomized block design consisting of 2 factors. The first factor was corn cobs biochar which was applied 1 week after basic fertilizer treatment and consisted of 4 levels, namely B0: without biochar, B1: 2.5 t ha-1, B2: 5 t ha-1, and B3: 10 t ha-1. The second factor was the dosage of liquid of Trichoderma harzianum, namely TR0: without T.harzianum, TR1: 10 mL L-1, and TR2: 20 mL L-1, which was applied three times at 14, 28 and 42 days after planting. Data were analyzed using the F test and continued with DMRT (Duncan Multiple Range Test) at P= 0.05 level. The results showed that the application of 5 t biochar ha-1 was able to remove lead and decreased lead uptake in plants. Application of T. harzianum could remove and decrease absorption in plant tissue biochar was not able to increase the growth of shallot while T. harzianum increased the number of leaves and the number of tubers.

Author Biography

Okti Herliana, Universitas Jenderal Soedirman

Laboratory of Agroecology

References

Akan, J.C., Mohmoud, S., Yikala, B.S. and Ogugbuaja, V.O. 2012. Bioaccumulation of some heavy metals in fish samples from river Benue in Vinikilang, Adamawa state, Nigeria. American Journal of Analytical Chemistry 3:727–736 doi:10.4236/ajac.2012.311097.

Ali, Z., Nawaz, I., Yousaf, S., Naqvi, S.T.A., Mahmood, T., Khan, N. and Iqbal, M. 2019. Wheat straw biochar promotes the growth and reduces the uptake of lead, cadmium and copper in Allium cepa. International Journal of Agriculture and Biology 21: 1173‒1180.

Alia, N., Sardar, K., Said, M., Salma, K., Sadia, A., Sadaf, S., Toqeer, A. and Miklas, S. 2015. Toxicity and bioaccumulation of heavy metals in spinach (Spinacia oleracea) grown in a controlled environment. International Journal of Environmental Research and Public Health 12:7400–7416 doi:10.3390/ijerph120707400.

Aslam, Z., Khalid, M., Naveed, M. and Shahid, M. 2017. Evaluation of green waste and popular twigs biochar produced at different pyrolysis temperatures for remediation of heavy metals contaminated soil. International Journal of Agriculture and Biology 19: 1427‒1436.

Bahar YH. 2016. The impact of farmer behaviour in shallot cultivation on changing conditions agroecosystem in Brebes Regency. Jurnal Penyuluhan Pertanian 11(1):23-36 (in Indonesian).

Basuki. 2014. Problems identification and shallots farming analyze in the highland during the rainy season in Majalengka District. Jurnal Hortikultura, 24(3):266-275 (in Indonesian).

Chaturvedi, A.D., Pal, D., Penta, S. and Kumar, A. 2015. Ecotoxic heavy metals transformation by bacteria and fungi in aquatic ecosystem. World Journal of Microbiology and Biotechnology 31:1595–1603, doi:10.1007/s11274–015–1911–5.

Chen, Y., Hua, Y., Zhang, S. and Tian, G. 2005. Transformation of heavy metal forms during sewage sludge bioleaching. Journal of Hazardous Materials 123:196–202, doi:10.1016/j.jhazmat.2005.03.047.

Cho, Y., Bolick, J.A. and Butcher, D.J. 2009. Phytoremediation of lead with green onions (Allium fistulosum) and uptake of arsenic compounds by moonlight ferns (Pteris cretica cv Mayii). Microchemical Journal 91(1): 6-8, doi: 10.1016/j.microc.2008.05.008.

Cobbet, C.S. 2000. Phytochelatins and their roles in heavy metal detoxification. Plant Physiology 123: 825–832.

Damodaran, D., Suresh, G. and Mohan, R.B. 2011. Bioremediation of soil by removing heavy metals using Saccharomyces cerevisiae. 2nd International Conference on Environmental Science and Technology, IPCBEE vol.6 (2011), IACSIT Press, Singapore.

Darsan, S., Sulistiyaningsih, E. and Wibowo, A. 2016. Various shallot seed treatments with Trichoderma to increase growth and yield on sandy coastal. Jurnal Ilmu Pertanian (Agricultural Science)1 (3) : 94-99.

Dixit, R., Wasiullah, Malaviya, D., Pandiyan, K., Singh, U.B., Sahu, A., Shukla, R., Singh, B.P., Rai, J.P., Sharma, P.K., Lade, H. and Paul, D. 2015. Bioremediation of heavy metals from soil and aquatic environment: An overview of principles and criteria of fundamental processes. Sustainability 7: 2189–2212.

Fellet, G., Marmiroli, M. and Marchiol, L. 2014. Elements uptake by metal accumulator species grown on mine tailings amended with three types of biochar. Science of the Total Environment 468–469: 598–608

Flora, S.J.S., Flora, G., Saxena, G. and Mishra, M. 2007. Arsenic and lead induced free radical generation and their reversibility following chelation. Cellular and Molecular Biology 53: 26‒47.

Fu, F. and Wang, Q. 2011. Removal of heavy metal ions from wastewaters: A review. Journal of Environmental Management 92: 407–418.

Gelagutashvili, E. 2013. Comparative study on heavy metals biosorption by different types of bacteria. Open Journal of Metal 3: 62–67, doi:10.4236/ojmetal.2013.32A1008.

Ghelich, S., Zarinkamar, and Fatemeh. 2013. Histological and ultrastructure changes in Medicago sativa in response to lead stress. Journal of Pharmacognosy and Phytochemistry 2: 20-29.

Hadiawati, L., Suriadi, A., Sugianti, T. and Zulhaedar, F. 2020. Application of Trichoderma-enriched compost on shallot productivity and storability in East Lombok, West Nusa Tenggara. Jurnal Hortikultura (1): 57-64 (in Indonesian).

Hardiani, H. 2008. Clean up contaminated soil of hazardous waste from the deinking process in the paper industry by phytoremediation. Jurnal Riset Industri, 2(2):64-75 (in Indonesian).

Harms, H., Schlosser, D. and Wick, L.Y. 2011. Untapped potential: Exploiting fungi in bioremediation of hazardous chemicals. Nature Reviews Microbiology 9:177–192, doi:10.1038/nrmicro2519.

Hartini. 2011. Plumbum (Pb) levels in shallot bulbs in Kersana District, Brebes. Jurnal Visikes 10(1): 69-75 (in Indonesian).

Haryati, U. and Erfandi, D. 2019. Improvement of soil properties and yield of shallot (Allium cepa) using mulch and soil amendments. Jurnal Hortikultura 10(3): 200-2013 (in Indonesian).

Herliana, O., Soesanto, L. and Mawadah, E. 2018. Phytobioremediation of cadmium contaminated soil using combination of Ipomoea reptans Poir and Trichoderma sp. and its effect on spinach growth

and yield. Journal of Degraded and Mining Lands Management 6(1): 1519-1526, doi:10.15243/jdmlm. 2016.061.1519.

Hidayat, B. 2015. Remediation of heavy metal contaminated soil using biochar. Jurnal Pertanian Tropik 2(1):31-41 (in Indonesian).

Hussain, M., Farooq, M., Nawaz, A., Al-Sadi, A.M., Solaiman, Z.M., Alghamdi, S.S., Ammara, U., Ok, Y.S. and Siddique, K.H.M. 2017. Biochar for crop production: potential benefits and risks. Journal of Soils and Sediments 17: 685–716

Ippolito, J.A., Laird, D.A. and Busscher, W.J. 2012.Environmental benefits of biochar. Journal of Environmental Quality 41: 967–972

Karyadi, K., Syarifudin, S. and Soterisnanto, D. 2012. Accumulation of heavy metal lead (Pb) as pesticide residue in agricultural land (case study on shallot agricultural land in Gemuh District, Kendal Regency). Jurnal Ilmu Lingkungan 9(1): 1-9 (in Indonesian).

Komárek, M., Vaněk, A. and Ettler, V. 2013. Chemical stabilization of metals and arsenic in contaminated soils using oxides: a review. Environmental Pollution 172: 9–22.

Kopittke, P., Asher, C.J., Kopittke. R.A. and Menzies, N.W. 2007. Toxic effects of Pb2+ on growth of cowpea (Vigna unguiculata). Environmental Pollution 150: 280-287.

Kumar, G. and Tripathi, R. 2008. Lead-induced cytotoxicity and mutagenicity in grass pea. Turkish Journal of Biology 32: 73-78.

Kurniawan, A. and Nuraeni, E. 2016. Heavy metal mycoremediation: a review. Jurnal Bioteknologi dan Biosains Indonesia. 3(1): 36-45 (in Indonesian)

Latifah, A., Kustantinah, and Soesanto, L. 2011. Utilization of several isolates (Trichoderma harzianum) as biological control agents for fusarium wilt disease in shallots in planta. Jurnal Eugenia 17(2): 86-95 (in Indonesian).

Mohsenzadeh, F. and Shahrokhi, F. 2014. Biological removing of cadmium from contaminated media by fungal biomass of Trichoderma species. Journal of Environmental Health Science & Engineering 12:102.

Murtaza, G., Ghafoor, A., Qadir, M., Owens, G., Aziz, M.A., Zia, M.H. and Saifullah, 2010. Disposal and use of sewage on agricultural lands in Pakistan: A Review. Pedosphere 20: 23‒34.

Nurhasanah, Andrini, F. and Hamidy ,Y. 2015. Antifungal activity of red onion (Allium ascalonicum L.) juice against Candida albicans in vitro. Jurnal Ilmu Kedokteran 9 (2): 71-77 (in Indonesian).

Nurhidayati and Mariati. 2014. Utilization of maize cob biochar and rice husk charcoal as soil amendments for improving acid soil fertility and productivity. Journal of Degraded and Mining Lands Management 2(1): 223-230.

Nurhidayati, S., Majid, A. and Mihardjo, P.A. 2015. Utilization of liquid bio fungicide with Trichoderma sp. active compound to control anthracnose disease (Colletotrichum sp.) in chilli on the field. Berkala Ilmiah Pertanian 1(1): 19-27 (in Indonesian).

Ogunlade, M.O. and Agbeniyi, S.O. 2011. Impact of pesticides use on heavy metal pollution in cacao soil of Cross- River, Nigeria. African Journal of Agriculture Research 6(16): 3725 - 3728.

Park, J.H, Girish, K.C., Nanthi, S.B., Jae, W.C. and Thammared, C. 2011. Biochar reduces the bioavailability and phytotoxicity of heavy metals. Plant and Soil 348: 439 - 451, doi 10.1007/s11104- 011-0948-y.

Paz-Ferreiro, J., Lu, H., Fu, S., Méndez, A. and Gascó, G. 2014. Use of phytoremediation and biochar to remediate heavy metal polluted soils: a review. Solid Earth 5: 65–75.

Prayudi, M., Zubair, A. and Maricar, I. 2015. Phytoremediation of soil contaminated Cr metal with vetiver plant on composted soil media. Repository of UNHAS, http://repository.unhas.ac.id /bitstream/handle/123456789/18791/.

Priandoko, E.A., Parwanayoni, N.M.S. and Sundra, I.K. 2012. Heavy metal content (Pb and Cd) in green mustard (Brassica rapa) and Carrot (Daucus carrotafora) distributing in Denpasar City Market. Jurnal Simbiosis I (1) : 9-20 (in Indonesian).

Puga, A.P., Abreu C.A., Melo L.C.A. and Beesley L. 2015. Biochar application to a contaminated soil reduces the availability and plant uptake of zinc, lead and cadmium. Journal of Environmental Management 159: 86-93.

Rahayu, Mujiyo and Arini, R.U. 2018. Land suitability evaluation of shallot (Allium ascalonicum L.) at production centres in Losari District, Brebes. Journal of Degraded and Mining Lands Management 6(1): 1505-1511, doi:10.15243/jdmlm. 2018.061.1505.

Rao, D.V., Shivannavar, C.T. and Gaddad, S.M. 2002. Bioleaching of copper from chalcopyrite ore by fungi. Indian Journal of Experimental Biology 40:319–324.

Ratnasari, I.F.D., Hadi, S.N., Suparto, S.R., Herliana, O. and Ahadiyat, Y.R. 2020. Phytoremediation of cadmium-contaminated soil using terrestrial kale (Ipomoea reptans Poir) and corncob biochar. Journal of Degraded and Mining Lands Management 7(4): 2313-2318, doi: 10.15243/jdmlm. 2020.074.2313.

Rincon, A.M., Benitez, T., Codon, A.C. and Moreno-Mateos, M.A. 2009. Biotechnological aspects of Trichoderma spp. In: Rai, M. and Bridge, P.D. (eds.), Applied Mycology (pp. 216–238). London: CAB International. http://doi.org/10.1079/9781845935344.0216.

Rosidah, S., Anggraito, Y.U, and Pukan, K.K. 2014. Tolerance test of tobacco (Nicotiana tabacum L.) to cadmium (Cd), lead (Pb), and copper (Cu) stress in liquid culture. Journal of Life Sciences Unnes 3 (2): 68-77 (in Indonesian).

Santoso, S.E., Soesanto, L. and. Haryanto, T.A.D. 2007. Biological emphasis of moler disease in shallots using Trichoderma harzianum, Trichoderma koningii, and Pseudomonas fluorescens P60. Jurnal HPT Tropika 7(1): 53-61(in Indonesian).

Shoresh, M., Harman, G.E. and Mastouri, F. 2010. Induced systemic resistance and plant responses to fungal biocontrol agents. Annual Review in Phytopathology 48: 21–43.

Shovan, L.R., Bhuiyan, M.K.A., Begum, J.A. and Pervez, Z. 2008. In vitro control of Colletotrichum dematium causing anthracnose of soybean by fungicides, plant extracts and Trichoderma harzianum. International Journal of Sustainable Crop Production 3(3): 10–17.

Sukartono and Utomo, W.H. 2012. The role of biochar as a soil amendment in maize cultivation in semiarid tropical sandy loam Lombok. Buana Sains 12 (1) : 91-98 (in Indonesian).

Taufik, Y. 2015. Horticultural Production Statistics, 2014. Direktorat Jenderal Hortikultura Kementerian Pertanian(in Indonesian).

Tripathi, P., Singh, P.C., Mishra, A., Chauhan, P.S., Dwivedi, S., Bais, R.T. and Tripathi, R.D. 2013. Trichoderma: a potential bioremediator for environmental clean up. Clean Technologies and Environmental Policy 15(4): 541 – 550.

Vankar, P.S. and Bajpai, D. 2008 Phytoremediation of Chrome-VI of tannery effluent by Trichoderma species. Desalination 222: 255–262.

Wang, H., Lin, K., Hou, Z., Richardson, B. and Gan, J. 2010. Sorption of the herbicide terbuthylazine in two New Zealand forest soils amended with biosolids and biochars. Journal of Soils and Sediments 10: 283‒289.

Wani, P.A. and Ayoola, O.H. 2015. Bioreduction of Cr (VI) by heavy metal resistant Pseudomonas species. Journal of Environmental Science and Technology 8:122–130, doi:10.3923/jest.2015.122.130.

Widyatmoko, H. 2011. pH accuracy as a parameter of the level of heavy metal contamination in the soil. Jurnal Teknik Lingkungan 5(5): 173 – 178, 173 (in Indonesian).

Wulandari, R., Purnomo, T. and Winarsih. 2014. The ability of watercress plants (Ipomoea aquatica) in absorbing heavy metals cadmium (Cd) based on different concentrations and exposure times. LenteraBio 3(1): 83-89 (in Indonesian).

Zheng, W., Guo, M., Chow, T., Bennett, D.N., and Rajagopalan, N. 2010. Sorption properties of greenwaste biochar from two trizaine pesticides. Journal of Hazardous Materials 181 121-126.

Yuan, J.H., R.K. Xu, and H. Zhang. 2011. The forms of alkalis in the biochar produced from crop residues at different temperatures. Journal of Bioresource Technology 102: 3488–3497. doi:10.1016/j.biortech.2010.11.018.

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Submitted

31-01-2021

Accepted

05-03-2021

Published

01-04-2021

How to Cite

Herliana, O., Ahadiyat, Y. R., & Cahyani, W. (2021). Utilization of biochar and Trichoderma harzianum to promote growth of shallot and remediate lead-contaminated soil. Journal of Degraded and Mining Lands Management, 8(3), 2743–2750. https://doi.org/10.15243/jdmlm.2021.083.2743

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Section

Research Article