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Retno Wilujeng
International Research Centre for the Management of Degraded and Mining Lands, Brawijaya University.


Scopus ID : 6508116842
ORCID ID : 0000-0002-3955-1278 

Gusti Irya Ichriani

F Fahrunsyah

Yulia Nuraini

Eko Handayanto
IRC MEDMIND Brawijaya University

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The possible use of coal fly ash and phosphate-solubilizing fungi for improving the availability of P and plant growth in acid soil

Retno Wilujeng, Gusti Irya Ichriani, F Fahrunsyah, Yulia Nuraini, Eko Handayanto
  J. Degrade. Min. Land Manage. , pp. 2471-2480  
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The availability of P in acid soils may be raised through the application of phosphate solubilizing fungi (PSF). Coal fly ash (CFA) that has a high pH and contains a relatively high P may also be used to raise the availability of P in acid sois. The purpose of this study was to explore the possible use of CFA and PSF in a biochar-compost carrier to improve the availability of P and plant growth in acid soil. Combined applications of two PSF isolates (Aspergillus oryzae = F1, and Neosartorya fischeri = F2) carried in three levels of biochar-compost  (80% biochar + 20% compost = B1, 70% biochar + 30% compost = B2, and 60% biochar + 40% compost =B3), and two doses of CFA (60 t/ha = C1, and 80 t/ha = C2) were tested in this study through two experiments. The results of experiment 1 (laboratory experiment) showed that the application of N. fischeri carried in 70% biochar + 30% compost combined with 80 t CFA/ha (F2B2C2 treatment) significantly increased the available P more than other treatments. In comparison with control,  the increase of soil available P content ranged from 13% in the F1B1C1 treatment (A. oryzae in 80% biochar + 20% compost combined with 60 t CFA/ha) to 101% in the F2B2C2 treatment (N.fischeri in 70% biochar + 30% compost combined with 80 t CFA/ha). The results of experiment 2 (glasshouse experiment) showed that the highest dry weight of maize shoot was obtained by the F2B2C2 treatment that increased 123% compared to control. The highest P uptake by maize was obtained by the F2B2C2 treatment (N. fischeri in 70% biochar + 30% compost combined with 80 t CFA/ha).


biochar; coal fly ash; phosphate-solubilizing fungi; acid soil

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Acevedo, E., Galindo, C.T., Prada, F., Navia, M. and Romero, H.M. 2014. Phosphate-solubilizing microorganisms associated with the rhizosphere of oil palm (Elaeis guineensis Jacq.) in Colombia. Applied Soil Ecology 80(8): 26–33. doi: 10.1016/j.apsoil.2014.03.011.

Alori, E.T., Glick, B.R. and Babalola, O.O. 2017 Micro¬bial phosphorus solubilization and its potential for use in sustainable agriculture. Frontiers in Micro¬biology 8: 971, doi: 10.3389/fmicb.2017.00971.

Balemi, T. and Negisho, K. 2012. Management of soil phosphorus and plant adaptation mechanisms to phosphorus stress for sustainable crop produc¬tion: a review. Journal of Soil Science and Plant Nutrition 12(3): 547-562, doi: 10.4067/ S0718-95162012005000015.

Bharti, B., Matte, D.B., Badole, W.P. and Deshmukh, A. 2000. Effect of fly ash on yield, uptake of nutri¬ents and quality of green gram grown on a Vertisol. Journal of Soils and Crops 10: 122-124.

Bray, R.H. and Kurtz, L.T. 1945, Determination of total, organic, and available forms of phosphorus in soils. Soil Science 59: 39-45.

Budianta, D., Wiralaga, A.Y.A. and Wahana, L. 2010. Changes in some soil chemical properties of Ul-tisol applied by mulch from empty fruit bunches in an oil palm plantation. Journal of Tropical Soils 15(2): 111-118, doi: 10.5400/ jts.2010.15.2.111.

Chakkaravarthy, V.M., Arunachalam, R., Vincent, S., Paulkumar, K. and Annadurai, G. 2010. Biodegradation of tricalcium phosphate by phosphate-solubilizing bacteria. Journal of Biological Sciences 10(6): 531–535, doi: 10.3923/jbs.2010.531.535.

Ciccu, R., Ghiani, M., Serci, A., Fadda, S., Peretti, R. and Zucca, A. 2003. Heavy metal immobilization in the mining-contaminated soils using various industrial wastes. Mineral Engineering 16: 187-192.

Ciećko, Z., Żołnowski, A.C., Madej, M., Wasiak, G. and Lisowski, J. 2015. Long-term effects of hard coal fly ash on selected soil properties. Polish Journal of Environmental Studies 24(5): 1949-1957.

Cunningham, J.E. and Kuiack, C. 1992. Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii. Applied Environmental Microbiology 58(5): 1451–1458.

Das, B.K., Choudhury, B.H. and Das, K.N. 2013. Effect of integration of fly ash with fertilizers and FYM on nutrient availability, yield and nutrient uptake of rice in Inceptisols of Assam, India. International Journal of Advancements in Research & Technology 2(11): 190-208.

Fahrunsyah, F., Kusuma, Z., Prasetya, B. and Handayanto, E. 2018. Improvement of some chemical properties of an Ultisol of East Kalimantan through the application of combined coal fly ash and oil palm empty fruit bunch. Bioscience Research 15(3):1805-1814.

Farrell, M., Perkins, W.T., Hobbs, P.J., Griffith, G.W. and Jones, D.L. 2010. Migration of heavy metals in soil as influenced by compost amendments. Environmental Pollution 158(1):55–64.

Gorman, J.M., Sencindiver, J.C., Horvath, D.J., Singh, R.N. and Keefer, R.F. 2000. Erod¬ibility of fly ash used as a topsoil substitute in mine land reclamation. Journal of Environmental Quality 29: 805-811.

Haynes, R.J. and Mokolobate, M.S. 2001. Ameliora-tion of al toxicity and deficiency in acid soil by ad¬ditions of organic residues: A critical review of the phenomenon and the mechanisms involved. Nutri¬ent Cycling in Agroecosystems 59(1): 47-63.

Hermawan, A., Sabaruddin, M., Hayati, R. and Warsito. 2014. Changes in P absorption in Ultisols due to the application of a mixture of coal fly ash-chicken manure. Jurnal Ilmu Tanah dan Agroklimatologi 11(1): 1 – 10 (in Indonesian).

Ichriani, G.I., Syekhfani, Nuraini, Y. and Handayanto, E. 2017. Solubilization of inorganic phosphate-solubilizing fungi isolated from oil palm empty fruit bunches of Central Kalimantan. Bioscience Research 14(3): 705-712, doi: 10.12911/22998993/92891.

Ichriani, G.I., Syekhfani, Nuraini, Y. and Handayanto, E. 2018. Formulation of biochar-compost and phosphate-solubilizing fungi from oil palm empty fruit bunch to improve the growth of maize in anUltisol of Central Kalimantan. Journal of Ecological Engineering 19(6): 45-55, doi: 10.12911/22998993/92891.

Ifansyah, H. 2013. Soil pH and solubility of alu-minium, iron, phosphorus in ultisols: The role of humic acid. Journal of Tropical Soils 18(3): 203-208, doi: 10.5400/jts.2013.18.3.203.

Im-Erb, R., Bamroongrugsa, N., Kawashima, K., Amano, T. and Kato, S. 2004. Utilization of coal ash to improve acid soil. Journal of Science and Technology 26(5): 697-708

Jain, R., Saxena, J. and Sharma, V. 2010. The evaluation of free and encapsulated Aspergillus awamori for phosphate solubilization in fermentation and soil-plant system. Applied Soil Ecology 46: 90–94, doi: 10.1016/j.apsoil.2010.06.008.

Jala, S. and Goyal, D. 2006. Fly ash as a soil ameliorant for improving crop production - a review. Bioresource Technology 97: 1136-1147.

Khan, R.K. and Khan, M.W. 1996. The effect of fly ash on plant growth and yield of tomato. Environmental Pollution 92(2):105–111.

Lee, H., Ha, H.S., Lee, C.H., Lee, Y.B. and Kim, P.J. 2006. Fly ash effect on improving soil properties and rice productivity in Korean paddy soils. Bioresource Technology 97: 1490, 2006, doi: 10.1016/j.biortech.2005.06.020.

Mahale, N.K., Patil, S.D., Sarode, D.B. and Attarde, S.B. 2012. Effect of fly ash as an admixture in agriculture and the study of heavy metal accumulation in wheat (Triticum aestivum), Mung Bean (Vigna radiata), and Urad Beans (Vigna Mungo). Polish Journal of Environmental Study 221(6): 1713 – 1719.

Minardi, S., Harieni, S., Anasrullah, A. and Purwanto, H. 2017. Soil fertility status, nutrient uptake, and maize (Zea mays L.) yield following organic mat¬ters and P fertilizer application on Andisols. IOP Conference Series: Materials Science and Engi¬neering 193 (2017), doi:10.1088/1757- 899X/193/1/012054.

Muduli, S.D., Chaturvedi, N., Mohapatra, P., Dhal, N.K. and Nayak, B.D. 2014. Growth and physiologi¬cal activities of selected leguminous crops grown in carbonated fly ash amended soil. Greener Jour¬nal of Agricultural Sciences 4(3): 83-90, doi: 10.15580/GJAS.2014.3.021114104.

Olsen, S.R. and Sommers, L.E. 1982. Phosphorus. In: Page, A.L. (ed.), Methods of Soil Analysis: Part 2. Chemical and Microbiological Properties. ASA Monograph 9: 403–430.

Panda, S.S., Mishra, L.P., Muduli, S.D., Nayak, B.D. and Dhal, N.K. 2015. The effect of fly ash on vegetative growth and photosynthetic pigment con¬centrations of rice and maize. Biologija 61(2): 94-100, doi: 10.6001/biologija.v61i2.3143.

Pathan, S.M., Aylmore, L.A.G. and Colmer, T.D. 2003. Soil properties and turf growth on a sandy soil amended with fly ash. Plant and Soil 256: 103-114.

Priatmadi, B.J., Saidy, A.R. and Septiana, M. 2014. Effects of coal fly ash on changes in soil chemical properties of South Kalimantan. Buana Sains 14(2): 1-6 (in Indonesian).

Ram, L.C., Tripathi, P.S.M. and Mishra, S.P. 1995. Moessbauer spectroscopic studies on the transformations of Fe-bearing minerals during combustion of coal: correlation with fouling and slagging. Fuel Process Technology 42:47-60.

Rani, K. and Kalpana, S. 2010. Utilization in agricultural and related field; a better alternative for eco-friendly maintenance of coal fly ash. Journal of Chemical and Pharmaceutical Research 2(5): 365-372.

Rautaray, S.K., Ghosh, B.C. and Mittra, B.N. 2003. Effect of fly ash, organic wastes and chemical fertilizers on yield, nutrient uptake, heavy metal content and residual fertility in a rice–mustard cropping sequence under acid lateritic soils. Bioresource Technology 90(3):275-283

Rayment, G.E. and Higginson, F.R. 1992. Australian laboratory handbook of soil and water chemicals methods. Australian Soil and Land Survey Handbook. Inkata Press, Melbourne, Sidney.

Reddy, T.P., Umadevi, M. and Rao, P.C. 2010. Effect of fly ash and farmyard manure on soil properties and yield of rice grown on an Inceptisol. Agricul-tural Science Digest 30(4): 281-285.

Sajwan, K.S., Paramasivam, S., Alva, A.K., Adriano, D.C. and Hooda, P.S. 2003. Assessing the feasibility of land application of fly ash, sewage sludge and their mixtures. Advances in Environmental Research 8(1): 77-91.

Scervino, J.M., Mesa, M.P., Mónica, I.D., Recchi, M., Moreno, S. and Godeas, A. 2013. Soil fungal isolates produce different organic acid patterns involved in phosphate salts solubilization. Biology and Fertility of Soils 49(6): 779–779, doi:10.1007/s00374-010-0482-8.

Sharma, S.K. and Kalra, N. 2006. Effect of fly ash incorporation on soil properties and productivity of crops: a review. Journal of Scientific and Industrial Research 65: 383 – 390.

Subagyo, H., Suharta, N. and Siswanto, A.B. 2000. Agricultural Soils in di Indonesia. Management of Land Resources of Indonesia. Indonesian Soil and Agroclimatology Research Centre, Bogor. pp 21-66 (in Indonesian).

Tan, K.H. 2003. Humic Matter in the Soil and the Environment: Principles and Controversies. Marcel Dekker, Inc. new York. USA.

Tripathi, R.C., Masto, R.E. and Ram, L.C. 2009. Bulk use of pond ash for cultivation of wheat– maize–eggplant crops in sequence on a fallow land. Resources, Conservation & Recycling 54:134-139.

Walkley, A. and Black, I.A. 1934. An examination of the Degtjaref method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Science 37:29-38.

Wilujeng, R. and Handayanto, E. 2019. Improvement of maize production on an Ultisol using coal fly ash and compost of oil palm empty fruit bunches. Jurnal Tanah dan Sumberdaya Lahan 6(1): 1043-1054, doi: 10.21774/ub.jtsl.2019.006.1.3 (in Indonesian).

Wong, M.H. and Wong, J.W.C. 1986. Effects of fly ash on soil microbial activity. Environmental Pollution Serie A 40:127–144.

Zhang, Y., Chen, F.S., Wu, X.Q., Luan, F.G., Zhang, L.P., Fang, X.M., Wan, S.Z., Hu, X.F. and Ye, J.R. 2018. Isolation and characterization of two phosphate-solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments. PLoS One 13(7): e0199625, doi: 10.1371/journal.pone.0199625.


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