Characterization of phosphate solubilizing bacteria isolated from Pb contaminated soils and their potential for dissolving tricalcium phosphate
Phosphorus solubilizing bacteria (PSB) enhances P availability in soils through dissolving inorganic P pools. The characteristics of PSB and the potential of three Pb tolerant phosphate solubilizing rhizobacteria, i.e. Pseudomonassp, Bacillus sp., and Actinomycites sp. were evaluated. PSB were isolated from soil samples contaminated with 300 and 500 mg Pb/kg after incubation for 30 days. Phosphate solubilizing bacteria were screened on for phosphate solubilisation ability in Pikovskaya agar medium (PA). In addition, two of the three indentified PSB strains (Pseudomonas sp. and Bacillus sp.) were characterized for their ability to solubilize tricalcium phosphate in Pikoskaya broth (PB) and also were examined their growth during culture medium incubation. The isolates exhibited different phosphate solubilization index, ranging from 1.87 to 2.98. Pseudomonas sp. had the highest ability to solubilize tricalcium phosphate: 9.82 mg P/ L and 12.23 mg P/ Lin Pikoskaya broth following the addition of 4 mg Pb/L and 2 mg /Pb L, respectively.
Bolan, N.S., Adriono, D.C., Naidu, R. 2003. Role of phosphorus in (im)mobilization and bioavailability of heavy metals in the soil-plant system. Reviews of Environmental Contamination and Toxicology 177:1-44.
Cao, X., Ma, L.Q., Singh, S.P., and Zhou, Q. 2008. Phosphate-induced lead immobilization from different lead minerals in soil under varying pH conditions. Environmental Pollution 152: 184 – 192.
Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, E.T., Lai, W.A., and Young, C.C. 2006. Phophate colubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34:33-41
Dawes, B.E. 1990. Lead. In Alloway, B.J. (ed) Heavy Metals in Soils. Pp 177-194. Hallsted Press. John Wiley & Son, Inc. New York.
Geesey, G. and Jang, L. 1990. Extra cellular ppolymers for metal binding. In H.L. Enrlrich and C.L. Brierly (eds): Microbial Mineral Recovery. pp. 223-337. MacGraw Hill. New York.
Hoa, T.T.H, Liemleam, W. and Annachhatre, A.P. 2007. Lead removel trough biological sulfat reduction process. Bioresource Technology 98: 2538-2548
Joseph, B., Ratra, R.R. and Lawrence, R. 2007. Characterization of plant growth promoting rhizobacteria associated with Chickpea (Cicer arietinum L.). International Journal of Pollutant Production 1:141-152.
Metting, F.B. 1999. Structure and physiological ecology of soil microbial communities. In. F.B. Metting (ed): Soil Microbial Ecology. pp 3-26. Marcel Dekker, Inc, New York.
Niswati, A., Yusnaini,S. and Arif, M.A.S. 2007. Phophate solubilizing microorganisms and available P on the rhizosphere of some ages and distance from the center of maize roots. Jurnal Tanah Tropika 13: 123-130
Park, J.H, Bolan, N., Megharaj, M., Naidu, R. and Chung, J.W. 2011. Bacterial assisted immmobilization of lead in soils: implictions for remediation. Pedologist 162-174
Park, J.H., Bolan, N., Megharaj, M. and Naidu, R. 2010. Isolation of phophate solubilizing of bacteria and charasterization of their effects on lead immobilization. Pedologist 67-75.
Premono, M.,E.,. Widayati, W.E. and Sumowijardjo, S. 2002. Populace dynamics of rhizopseudomonas on root surface of sugarcane. Jurnal Hayati 9 (2):55-58.
Raone, T.M. 1999. Lead resistence in two bacterial isolates from heavy metal- contamined soils. Microbial Ecology 37:218 – 224.
Rodriguez, H and Fraga, R. 1999. Phophate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advance 17: 319-339
Saraswati, R. 2004. Recent advances in understanding soil microbial activity and soil quality relationship. Jurnal Mikrobiologi Indonesia 2: 1-8.
Sharma, S., Kumar, V and Tripathi, R.B. 2011. Isolation of phophate solubilizing microorganism (psms) from soil. Journal of Microbiology and Biotechnology Research 1(2): 90-95.
Srivastava, S., Kausalya, M.T, Archana, G., Rupela, O.P. and Kumar, N.G. 2003. Efficacy of organic acid secreting bacteria in solubilization of rock phosphate in acidic alfisols. First International Meeting on Microbial Phosphate Solubilization, p 117-124. UN Grants Commission. India.
Subowo G., Ratmini, N.P.S, Dewi, D.S., and. Kentjanasari, A. 2004. Efektivitas Teknologi Remediasi Tanah Tercemar Logam Berat Pb dan Cd. Prosiding Seminar Inovasi Teknologi Sumber Daya Tanah dan Iklim.
- There are currently no refbacks.
Copyright (c) 2014 Journal of Degraded and Mining Lands Management
License URL: http://jdmlm.ub.ac.id/index.php/jdmlm/about/submissions#privacyStatement