Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer
DOI:
https://doi.org/10.15243/jdmlm.2016.041.697Keywords:
bacteria, biofertilizer, exopolysaccharide, indigenousAbstract
Steady soil aggregationis important for agricultural land which is formed by the micro-aggregate to become a macro-aggregate. This formation is mediated by organic material and various kinds of macro-organisms such as fungi, worms, ants and insects. An organic agencyinvolved in soil aggregation stabilityis exopolysaccharide (EPS) derived from bacterial, fungal mycelium, and products synthesized by plants. However, the use of EPS producing microorganisms as a biofertilizer has not been reported. This study was aimedto explore indigenous EPS-producing bacteria to solidify soil aggregationpotential for biofertilizer. Bacterial strains were isolated from soilsofthree regions at Malang East-Java; two areas of green bean plantation in Kendal Payak and Jambe Gede, as well as forest land. Soil samplewas derived from forest had hasa total bacteria population of 9.3 x 1011 CFU/mL.While soil samples from area Kendal Payak and Jambe Gede had total bacteria population of 1.5 x 109 CFU/mL and 2.4 x 109 CFU/mL, respectively. We selected three bacteria that could potentially produce abundant slime, namely as SPE-2, SPE-10 and SPE-20. The three selected bacteria are potential for biofertilizer because oftheir abundant slime, no antagonism and no symtoms as pathogen.
References
Arfarita, N., Djuhari, D. and Prasetya, B. 2016. The application of trichoderma viride strain frp 3 for biodegradation of glyphosate herbicide in contaminated land. Agrivita Journal of Agricultural Science 38(3): 275-281.
Bertin, C., Yang, X. and Weston, L.A. 2003. The role of root exudates and allelochemicals in the rhizosphere. Plant and Soil 256: 6783.
Bronick, C.J. and Lal, R. 2005. Soil structure and management: a review. Geoderma 124: 322.
Emtiazi, G., Ethemadifar,Z.and Habibi, M.H. 2004. Production of extrcellular polymer in azotobacter and biosorption of metal by exopolimer. African Journal of Biotchnology 3(6):330-333.
Flemming, H.C. and Wingender, J. 2001. Relevance of microbial extracellular polymeric substances (EPSs)-parts I: structural and ecological aspects. Water Science Technology 43:1–8.
Goenadi, D.H. 1995. Nutrient-solubilizing and aggregate-stabilizing microbes isolated from selected humic tropical soils. Menara Perkebunan 63(2): 60-66.
Hattori, T. 1988. Soil aggregates inmicrohabitats of microorganisms. Report of the Institute of Agricultural Research Tohoku University 37: 2336.
Iqbal, A., Bhatti, H.N., Nosheen, S., Jamil, A. and Malik, M.A. 2002. Histochemical and physicochemical study of bacterial exopolysaccharides. Biotechnology1(1): 2833.
Kumon, H., Tomochika, K., Matunaga, T., Ogawa, M. and Ohmori, H.A. 1994. Sandwich cup method for the pentertation assay of antimicrobial agents through Pseudomonas exopolysaccharides. Microbiol Immunology 38:615–619.
Lynch, J.M. and Bragg, E. 1985. Micro-organisms and soil aggregate stability Advances in Soil Science 2:133-171.
Mu’minah, B., Zubair, H. and Fachruddin. 2015. Isolation and screening bacterial exopplysaccharide (EPS) from potato rhizosphere in highland and the potential as a producer indole acetic acid (IAA). Procedia Food Science 3: 74-81.
Nuraini, Y., Arfarita, N. and Siswanto, B. 2015. Isolation and characteristic of nitrogen-fixing bacteria and phosphate-solubilizing bacteria from soil high in mercury in tailings and compost areas of artisanal gold mine. Agrivita Journal of Agricultural Science 37(1): 1-7.
Suntoro, 2003. Role of Organic Materials on Soil Fertility and Management Efforts. Sebelas Maret University Press. Surakarta
Wingender, J., Neu, T.R. and Flemming, H.C.1999. What are bacterial extracellularpolymeric substances? In: Wingender,J., Neu, T.R. and Flemming, H.C. (Eds),Microbial extracellular polymericsubstances: characterization, structure and function. Springer-Verlag, Berlin.115p.
Downloads
Submitted
Accepted
Published
How to Cite
Issue
Section
License
Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis) that it is not under consideration for publication elsewhere; that if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher. 
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Scientific Journal by Eko Handayanto is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at https://ub.ac.id.
Permissions beyond the scope of this license may be available at https://ircmedmind.ub.ac.id/.
This work is licensed under a 