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Nasrul Harahap
ORCID iD https://orcid.org/0000-0001-8918-0762
graduate student Bogor Agricultural Universty
Indonesia

Dwi Andreas Santosa
Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University, Bogor
Indonesia

Nuni Gofar
Department of Soil Science, Faculty of Agriculture, Sriwijaya University, Palembang
Indonesia

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The potential of exopolysaccharide-producing bacteria from rhizosphere of rubber plants for improving soil aggregate

Nasrul Harahap, Dwi Andreas Santosa, Nuni Gofar
  J. Degrade. Min. Land Manage. , pp. 1275-1281  
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Abstract


 This study aimed to examine the effect of bacteria found in the rhizosphere of rubber plants in producing exopolysaccharides to improve aggregate stability of sandy soils. Samples of soil have been taken in rhizosphere of rubber plants in West Kalimantan. Serial soil samples were diluted and cultured on ATCC no.14 medium to select potential bacteria to produce exopolysaccharides. Forty-five isolates of exopolysaccharide-producing bacteria isolated from the rhizosphere of rubber plants was inoculated on ATCC no.14 medium. Based on the observations of morphological colony of these isolates, most of them had similarities in colour and shape so that only ten different isolates were obtained based on the morphological colony. Ten isolates were re-grown on MacConcey medium. Three isolates formed thick or slimy mucus when cultured on MacConcey medium. Three isolates grown on the medium of ATCC 14 resulted in dry weight of exopolysaccharide (mg/mL) varying from 0.28 to 7.59 mg/mL with sucrose and glucose as carbon sources. The results of the molecular identification of the three isolates of Klebsiella sp. LW-13, Klebsiella pneumoniae strain DSM 30104 and Burkholderia anthina strain MYSP113 showed that Klebsiella sp. LW-13 and Burkholderia anthina strain MYSP113 with 2% organic matter increased soil aggregate stability from highly unstable (30.67%) to unstable (45.01-48.20%). This aligned with the results by scanning electron microscopy (SEM) on treated soil and without bacteria treatments.  

Keywords


exopolysaccharides,Sandy soil, rubber, bacteria,soil aggregate

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References


Alami, Y., Achouak, W., Marol, C. and Heulin, T. 2000. Rhizosphere soil aggregation and plant growth promotion of sunflowers by an exopolysaccharide-producing Rhizobium sp strain isolated from sunflower roots. Applied Environmental Microbiology 66(8): 3393-3398.

Alves, V.D., Freitas, F., Torres, C.A.V,, Cruz. M,, Marques. R, and Grandfils, C. 2010. Rheological and morphological characterization of the culture broth during exopolysaccharide production by Enterobacter sp. Carbohydrate Polymers 81: 758–764.

De Leenheer, L. and De Boodt, M.F.1959. Determination of aggregate stability by the change in mean weight diameter. In: Proceeding of International Symposium on Soil Structure, Ghent. Belgium.

Dlamini, A.M., Peiris, P.S., Bavor, J.H. and Kailasapathy, K. 2009. Rheological characteristics of an exopolysaccharide produced by a strain of Klebsiella oxytoca. Journal of Bioscience and Bioengineering 107: 272–274.

Emtiazi, G., Ethemadifar, Z. and Habibi, M.H. 2004. Production of extracellular polymer in Azotobacter and biosorption of metal by exopolymer. African Journal of Biotechnology 3(6): 330-333.

Eneje, R.C., Oguike, P.C. and Osuaku, S.2007. Temporal variations inorganic carbon, soil reactivity and aggregate stability in soils of contrasting cropping history. African Journal of Biotechnology 6(4): 369-374.

Enriquez, G.L., Saniel, L.S., Matias, R.R. and Garibay, G.1995. General Microbiology Laboratory Manual. Diliman: University of the Philippines Press.

Feng, L., Li, X., Duc, G. and Chen, J. 2009. Characterization and fouling properties of exopolysaccharide produced by Klebsiella oxytoca. Bioresource Technology 100: 3387–3394.

Freitas, F., Alves,V.D,, Torres, C.A.V., Cruz, M., Sousa, I., Melo, M.J.,Ramos, A.M. and Reis M.A.M. 2011. Fucose-containing exopolysaccharide produced by the newly isolated Enterobacter strain A47 DSM 23139. Carbohydrate Polymers 83: 159-165.

Imran, M.M., Reehana, K.A., Jayaraj, A, Ahamed, P.D., Dhanasekaran, N., Naif T.S. and Muralitharan, A.G.2016. Statistical optimization of exopolysaccharide production by Lactobacillus plantarum NTMI05 and NTMI20. Journal of Biological Macromolecules 66:213-225.

Kusuma, C.A., Wicaksono, K.S. and Prasetya, B. 2016. Improvement of physical properties and chemistry of sandy soils through application of Lactobacillus fermentum bacteria. Jurnal Tanah dan Sumberdaya Lahan 3(2): 401-410 (in Indonesian).

Mu'minah, Baharuddin, Subair, H. and Fahruddin. 2015. Isolation and screening Bacterial Exopolysaccharide (EPS) from potato rhizosphere in highland and the potential as a producer Indole Acetic Acid (IAA). Procedia Food Science (3): 74 –81.

Prasertsan, P., Dermlim, W., Doelle, H. and Kennedy, J.F. 2006. Screening, characterization and flocculating property of carbohydrate polymer from newly isolated Enterobacter cloacae WD7. Carbohydrate Polymers 66:289–297.

Prasertsan, P., Wichienchot, S., Doelle, H. and Kennedy, J.F. 2008. Optimization for biopolymer production by Enterobacter cloacae WD7. Carbohydrate Polymers 71:468-475.

Rachman, A. and Abdurachman, A. 2006. Determination of Soil Aggregate Stability. In Kurnia, U., Agus, F., Abudarachman, A. and Dariah, A. (eds.). Physical Properties of Soil and Analysis Method. Balai Besar Litbang Sumberdaya Lahan Pertanian, Bogor, 63- 74 (in Indonesian).

Ratto, M., Verhoef, R., Suihko, M.L., Blanco, A., Schols, H.A. and Voragen, A.G.J. 2006. Colanic acid is an exopolysaccharide common to many Enterobacteria isolated from paper machine slimes. Journal of Industrial Microbiology and Biotechnology 33: 359–367.

Remel. 2005. Microbiology Products: Instructions for use of MacConkey Agar. http://www.remelinc.com. [20 Jun 2016].

Santi, L,P., Dariah, Ai. and Goenadi, D.H. 2008. Increased stability of mineral soil aggregates by Exopolysaccharide-producing bacteria. Menara Perkebunan 76(2): 92-102 (in Indonesian).

Santi, L.P., Sudarsono, Goenadi, D.H., Murtilaksono, K. and Santosa, D.A. 2010. Effect of application of Burkholderia cenocepacia inoculum and organic matter on physical properties of sandy soils. Menara Perkebunan 78(1): 9-18 (in Indonesian).

Soil Survey Staff.1993. Soil Survey Manual. USDA handbook No. 18.Washington.USA.

Steel, R.G.D. and Torrie, J.H. 1980. Principles and Procedures of Statistics. A Biometrical Approach. 2nd ed. McGraw-Hill. New York.

Tallgren, A.H., Airaksinen, U., von Weissenberg, R., Ojamo, H., Kuusisto, J. and Leisola, M. 1999. Exopolysaccharide-producing bacteria from sugar beets. Applied Environmental Microbiology 65(2):862-864.

Torres, C.A.V., Marques, R., Antunes, S., Alves, V.D., Sousa, I., Ramos, A.M., Oliveira, R., Freitas, F. and Reis, M.A.M., 2012. Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter A47 using multivariate statistical analysis. Journal of Biotechnology 119: 148–156

Yatno, E. 2011. Role of organic materials in improving soil physical quality and plants production. Jurnal Sumberdaya Lahan 5 (1) : 175-182.


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