Tolerance mechanisms in mercury-exposed Chromolaena odorata (l.f.) R.M. King et H. Robinson, a potential phytoremediator


  • H J P Alcantara School of Chemisty, University of Melbourne
  • G C Rivero School of Chemisty, University of Melbourne
  • J M Puzon School of Chemisty, University of Melbourne



antioxidants, Chromolaena odorata, mercury, phytoremediation


Chromolaena odorata (L.f.) R.M. King et H. Robinson plants were grown in Hoagland’s solutions with 0.00 ppm and 1.00 ppm Hg(NO3)2. The calcium, magnesium, iron, and sulfur levels in the leaves were found to be not significantly affected by presence of the uptaken Hg2+. The chlorophyll a, chlorophyll b, and total chlorophyll contents of its leaves also remained within normal levels, which may indicate that the photosynthetic machinery of the Hg-exposed C. odorata was unaffected by the presence of Hg2+. The results of the ICP-AES analyses of the Hg2+ contents established the presence of Hg2+ in all the subcellular components obtained from the leaves of the Hg-treated C. odorata plants, and that the ultimate localization of Hg2+ is in the vacuoles. The findings revealed no significant differences in the degree of oxidative injury between the cells from the control and Hg-treated plants, as evidenced by the low lipid peroxidation levels obtained with the TBARS assay. The SH-containing biomolecules that were initially detected through DTNB assay manifested a predominant peak in the RP-HPLC chromatographs of both the control and Hg-treated plants, with their retention times falling within the ranges of GSH, MT, and cysteine standards. However, the concentrations of the GSH- and/or MT-like, Cys-containing biomolecules detected in the leaves of Hg-treated C. odorata plants were ten times higher than those of the control.The findings of this study suggest that the enhanced antioxidative capacity, the production of Hg-binding biomolecules, and the localization of Hg2+ ions ultimately in the vacuoles of the leaves are the mechanisms which bring about Hg2+ tolerance and homeostasis in C. odorata plant. These results indicate that C. odorata is a potentially effective phytoremediator for Hg2+.

Author Biographies

H J P Alcantara, School of Chemisty, University of Melbourne


G C Rivero, School of Chemisty, University of Melbourne


J M Puzon, School of Chemisty, University of Melbourne



Alloway, B. and Ayres, D. 1993. Heavy metals. In: Chemical Principles of Environmental Pollution, pp. 140-164. Chapman and Hall, Great Britain.

Arnon, D. 1949. Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris. Plant Physiology 24(1): 1-14.

Chaney, R., Malik, M., Li, Y., Brown, S., Brewer, E., Angle, J. and Baker, A. 1997. Phytoremediation of Soil Metals. Current Opinion in Biotechnology 8: 279–284.

Cho, U. and Park, J. 2000. Mercury-induced oxidative stress in tomato seedlings. Plant Science 156: 1–9.

Cobbett, C. and Goldsbrough, P. 2002. Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis. Annual Review of Plant Biology 53: 159-182.

De Vos, R., Vonk, M., Vooijs, R. and Schat, H. 1992. Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silene cucubalus. Plant Physiology 98: 853–858.

Gallego S., Benavides M. and Tomaro, M. 1996. Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress. Plant Science 121: 151-159.

Grill, E., Winnacker, E. and Zenk, M. 1987. Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins. Proceedings of the National Academy of Sciences U.S.A. 84: 439-443.

Gupta, M., Tripathi, R., Rai, U. and Chandra, P. 1998. Role of glutathione and phytochelatin in Hydrilla verticillata (L.f.) Royle and Vallisneria spiralis L. under mercury stress. Chemosphere 37: 785-800.

Hernandez, L., Lozano-Rodriguez, E., Garate, A. and Carpena-Ruiz, R. 1998. Influence of cadmium on the uptake, tissue accumulation, and subcellular distribution of manganese in pea seedlings. Plant Science 132: 139-151

Hoagland, D.R. and Arnon, D.I. 1938. The water-culture method of growing plants without soil. University of California Agricultura Experimental Station Circural 347: 1-39.

Iglesia-Turiño, S., Febrero, A., Jauregui, O., Caldelas, C., Araus, J. and Bort, J. 2006. Detection and Quantification of Unbound Phytochelatin 2 in Plant Extracts of Brassica napus Grown with Different Levels of Mercury. Plant Physiology 142: 742-749.

Josue, R., Puzon, J., Rivero, G. and Villanueva, J. 2006. Glutathione Mediates Mercury Binding and Detoxification. In Ipomoea aquatica Forsk. Proceedings of the 8th International Conference on Mercury as a Global Pollutant, Madison, Wisconsin, U.S.A. p.115.

Josue, R., Villanueva, J. and Rivero, G. 2004. Mercury uptake and phytochelatin production in Ipomoea aquatica Forsk. The Philippine Agricultural Scientist 87(3): 312-321.

Kabata-Pendias, A. and Pendias, H. 1986. Mercury. In: Trace Elements in Soils and Plants, pp. 116-125. CRC Press, Inc., Boca Raton, Florida, USA.

Kubota, H., K. Sato, T. Yamada, and T. Maitani. 2000. Phytochelatin homologs induced in hairy roots of horseradish. Phytochemistry 53: 239-245.

Lintongan, P., Cariño, F., and G. Rivero. 2004. Photosynthetic protein from Chlorella vulgaris Strain Bt-09 may be responsible for the coping mechanism against cadmium toxicity. Bulletin of Environmental Contamination and Toxicology 72: 1232-1239

Maitani, T., Kubota, H., Saio, K. and Yamada, T. 1996. The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. PIant Physiology 110: 1145-1150.

Matoh, T., Watanabe, J. and Takahashi, E. 1987. Sodium, potassium, chloride, and betaine concentrations in isolated vacuoles from salt-grown Atriplex gmelini leaves. Plant Physiology 84: 173-177.

Mehra, R., Miclat, J., Kodati, V., Abdullah, R., Hunter, T. and Mulchandani, P. 1996. Optical spectroscopic and reverse-phase HPLC analyses of Hg(II) binding to phytochelatins. Biochemical Journal 314: 73–82.

Moore, T. 1974. Characterization of Photosynthetic Pigments. Research Experiments in Plant Physiology: A Laboratory Manual. Springer-Verlag New York, Inc.: Heidelberg. 10-15.

Noctor, G. and Foyer, C. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49: 249-279.

Ortega-Villasante, C., Rellán-Alvarez, R., Del Campo, F., Carpena-Ruiz, R. and Hernández. L. 2005. Cellular damage induced by cadmium and mercury in Medicago sativa. Journal of Experimental Botany 56(418): 2239-51.

Patra, M. and Sharma, A. 2000. Mercury toxicity in plants. The Botanical Review 66(3): 379–421.

Peuke, A. and Rennenberg, H. 2005. Phytoremediation with transgenic trees. Zeitschrift für Naturforschung 60: 199-207.

Phan, T., Hughes, M., Cherry, G., Le, T. and Pham, H. 1996. An aqueous extract from the leaves of Chromolaena odorata (Formerly Eupatorium odoratum) (Eupolin) inhibits collagen lattice contraction by human dermal fibroblasts. The Journal of Alternative and Complementary Medicine 2: 335 – 343.

Phan,T., See, P., Lee, S. and Chan, S. 2001. Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage. Burns 27: 319-327.

Pollard A., Powell, K., Harper, F. and Smith, J. 2002. The genetic basis of metal hyperaccumulation in plants. Critical Reviews in Plant Sciences 21(6): 539– 66.

Prasad, K., Paradha Saradhi, P. and Sharmila, P. 1999. Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environmental and Experimental Botany 42: 1-10.

Puzon, J., G. Rivero, and M. Montaño. 2008. Vacuolar sequestration as a coping mechanism in cadmium-treated Eichhornia crassipes (Mart.) Solms. The Philippine Agricultural Scientist 91: 123-133.

Rama Devi, S. and M. Prasad. 1998. Copper toxicity in Ceratophyllum demersum L. (Coontail), a free floating macrophyte: Response of antioxidant enzymes and antioxidants. Plant Science 138:157–165.

Rao, K. and Stresty, T. 2000. Antioxidative parameters in the seedlings of pigeopea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Science 157: 113-128.

Rauser, W.E. 1999. Structure and function of metal chelators produced by plants: the case for organic acids, amino acids, phytin, and metallothioneins. Cell Biochemistry and Biophysics 31: 19-48.

Roder, W., Phengchanh, S. and Keobulapha, B. 1997. Weeds in slash-and-burn rice fields in Northern Laos. Weed Research 37(2): 111-119.

Salt, D., Smith, R. and Raskin, I. 1998. Phytoremediation. Annual Review of Plant Physiology and Plant Molecular Biology 49: 643-668.

Schützendubel, A. and Polle, A. 2002. Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany 53:351-1365.

Schützendubel, A., Schwanz, P., Teichmann, T., Gross, K., Langenfeld-Heyser, R., Godbold, D. and Polle, A. 2001. Cadmium-induced changes in antioxidative systems, H2O2 content, and differentiation in pine (Pinus sylvestris) roots. Plant Physiology 127: 887-892.

Shohael, A., Ali, M. and Hahn, E. 2007. Glutathione metabolism and antioxidant responses during Eleutherococcus senticosus somatic embryo development in a bioreactor. Plant Cell, Tissue and Organ Culture 89: 121-129.

Subhadra, A., Panda, K. and Panda, B. 1993. Residual mercury in seed of barley of methanesulfate, maleic hydrazide, methyl mercury chloride and mercury-contaminated soil. Mutation Research 300 (34):141–149.

Van Noordwijk, M., Hairiah, K., Partoharjan, S., Labios, R. and Garrity. D. 1996. Food crop-based production systems as sustainable alternatives for Imperata grasslands. Agroforestry systems 36(1): 5-82.

Velasco-Alinsug, M.P., Namocatcat, J.A. and Rivero, G.C. 2005a. Bioavailability of mercury to plants thriving in a mine tailings dumpsite. Swiss Federal Institute for Forest, Snow and Landscape Research WSL (ed.) 2005: Metal fluxes and stresses in terrestrial ecosystems. Abstracts. Birmensdorf, Swiss Federal Institute for Forest, Snow and Landscape Research WSL.

Velasco-Alinsug, M.P., Rivero, G.C. and Quibuyen, T.O. 2005b. Isolation of Mercury-binding Peptides in Vegetative Parts of Chromolaena odorata. Biosciences 60: 252-259

Vogeli-Lange, R. and Wagner, G. 1990. Subcellular localization of cadmium and cadmium-binding peptides in tobacco leaves. Plant Physiology 92: 1086- 1093.

Zenk, M.H. 1996. Heavy metal detoxification in higher plants – A review. Gene 179(1): 21–30.








How to Cite

Alcantara, H. J. P., Rivero, G. C., & Puzon, J. M. (2013). Tolerance mechanisms in mercury-exposed Chromolaena odorata (l.f.) R.M. King et H. Robinson, a potential phytoremediator. Journal of Degraded and Mining Lands Management, 1(1), 09–20.



Research Article