Exploration and characterization of exopolysaccharide-producing bacteria from soil in West Kalimantan for improving sandy soil aggregation

Nasrul Harahap; Dwi Andreas Santosa; Nuni Gofar

doi:10.15243/jdmlm.2018.054.1373

Exploration and characterization of exopolysaccharide-producing bacteria from soil in West Kalimantan for improving sandy soil aggregation

Nasrul Harahap, Dwi Andreas Santosa, Nuni Gofar

J. Degrade. Min. Land Manage. , pp. 1373-1379

Viewed : 453 times

DOI https://doi.org/10.15243/jdmlm.2018.054.1373

Abstract

Exopolysaccharide (EPS) is a complex mix of macro-molecular electrolyte contained in the outer cells of bacteria excreted as mucus and has a role in soil aggregation. This study aims to obtain bacteria that have a high potential for exopolysaccharide-producing bacteria. A total of 112 isolates of exopolysaccharide-producing bacteria were isolated from rubber soil rhizosphere, secondary forest, and shrubs in PT. Hutan Ketapang Industri is the result of isolation on ATCC no.14 medium. Based on the observations of a morphological colony of these isolates, most of them similarities of color and shape but only 25 colonies are different isolates were obtained based on colony morphology. However, only 10 isolates formed a thick mucus or slimy when cultured on MacConcey agar. the results show that obtained three isolates of exopolysaccharide-producing bacteria have a higher value of the dry weight i.e. isolates RB292 (7.53 mg/mL) followed by RB51 (7.55 mg/mL), and RB241 (1.75 mg/mL) with 2% sucrose. Isolates RB51 and RB292 increasing significantly soil aggregate stability at 2% dosage of organic matter with soil aggregate stability index from 30.61% to 47.87% and 45.79%. Homology of the isolates with known bacteria i.e. isolate RB51 was 98.86% homolog with Klebsiella sp. LW-13, isolate RB241 was 98.65% homolog with Klebsiella pneumonia strain DSM 30104 and isolate RB292 was 98.83% homolog with Burkholderia anthina strain MYSP113

Keywords

bacteria; exopolysaccharide; rhizosphere; soil aggregate stability

Full Text:

PDF

References

Bertin, C., Yang, X. and Weston, L.A.2003. The role of root exudates and allelochemicals in the rhizosphere. Plant and Soil 2(6):67–83.

De Leenheer, L. and De Boodt, M.1959. Determination of aggregate stability by the change in mean weight diameter. In: Proceeding of Internatational Symposium on Soil Structure, Ghent. Belgium.

Emtiazi, G., Ethemadifar, Z. and Habibi, M.H. 2004. Production of extracellular polymer in Azotobacter and biosorption of metal by exopolymer. African Journal of Biotechnology 3(6): 330-333.

Eneje, R.C., Oguike, P.C. and Osuaku, S. 2007. Temporal variations inorganic carbon, soil reactivity and aggregate stability in soils of contrasting cropping history. African Journal of Biotechnology 6(4): 369-374.

Enriquez, G.L., Saniel, L.S., Matias, R.R. and Garibay, G.1995. General Microbiology Laboratory Manual. Diliman: University of the Philippines Press.

Fairhurst, T. and McLaughlin, D. 2009. Sustainable Oil Palm Development on Degraded Land in Kalimantan. WWF, United States of America. 22p.

Hardjowigeno, S.1993. Soil Classification and Pedogenesis First Edition. Publisher Akademika Pressindo. Jakarta.

Imran, M.M., Reehana, K.A., Jayaraj, A., Ahamed, P.D., Dhanasekaran, N., Naif, T.S. and Muralitharan, A.G. 2016. Statistical optimization of exopolysaccharide production by Lactobacillus plantarum NTMI05 and NTMI20. Journal of Biological Macromolecules 66:213-225.

Kusuma, C.A., Wicaksono, K.S. and Prasetya B. 2016. Improvement of physical properties and chemistry of sandy sandy soils through application of Lactobacillus fermentum bacteria. Jurnal Tanah dan SumberdayaLahan 3(2): 401-410 (in Indonesian).

Moreno, J., Garcia, C.V., Lopez, M.J. and Serrano, G.S. 1999. Growth and exopolysaccharide production by Azotobacter vinelandii in media containing phenolic acids. Journal Applied Microbiology 86(3): 439-445.

Mu'minah, Baharuddin, Subair, H. and Fahruddin. 2015. Isolation and screening Bacterial Exopolysaccharide (EPS) from potato rhizosphere in highland and the potential as a producer Indole Acetic Acid (IAA). Procedia Food Science (3): 74 –81.

Mu'minah, Baharuddin, Subair, H., Fahruddin and Darwisah, B. 2016. The potential of producing bacteria exopolysaccharide from rhizosphere potato for produce exopolysaccharide. Journal Sains and Technology 16 (2):101–106.

Pawar, S.T., Bhosale, A.A., Gawade, T.B. and Nale, T.R.2013. Isolation, Screening, and optimization of exopolysaccharide producing-bacterium from the saline soil. Journal of Microbiology and Biotechnology 3(3): 24-31

Prasertsan, P., Dermlim, W., Doelle, H. and Kennedy, J.F. 2006. Screening, characterization and ﬂocculating property of carbohydrate polymer from newly isolated Enterobacter cloacae WD7. Carbohydrate Polymers 66: 289–297

Prasertsan, P., Wichienchot, S., Doelle, H. and Kennedy, J.F. 2008. Optimization for biopolymer production by Enterobacter cloacae WD7. Carbohydrate Polymers 71:468-475

Remel. 2005. Microbiology Products: Instructions for use of MacConkey Agar. http://www.remelinc.com.[28 Jun 2017]

Santi, L.P., Dariah, Ai, and Goenadi, D.H. 2008. Increased stability of mineral soil aggregates by Exopolysaccharide-producing bacteria. Menara Perkebunan 76(2): 92-102

Serrato, R.V., Sassaki, G.L., Cruz, L.M., Pedrosa, F.O., Gorin, P.A.J. and Iacomini, M. 2006. Culture conditions for the production of an acidic exopolysaccharide by the nitrogen-fixing bacterium Burkholderia tropica. Journal of Microbiology 52(5):489-493.

Soil Survey Staff.1993. Soil Survey Manual. USDA Handbook No. 18.Washington.The USA.

Steel, R.G.D. and Torrie, J.H. 1980. Principles and Procedures of Statistics. A Biometrical Approach. 2nd ed. McGraw-Hill.New York.

Suharta, N. and Suratman. 2004. Characterization and Analysis of Land Resources for Regional Development in Eastern Indonesia, scale 1: 250,000. The Land Resource Research Project and the Participatory Agricultural Technology Assessment (PAATP). Pusat Penelitian Tanah dan Agroklimat.Balitan.

Suharta, N., Anda, M. and Suwandi, V. 1999. Survey and Mapping of Land Review South Kalimantan Province (First Part), scale 1: 250,000. Land Resource and Agroforestry Research and Development Project.Pusat Penelitian Tanah dan Agroklimat.Badan Litbang Pertanian.

Syarovy, M., Ginting, E.N., Wiratmoko, D. and Santoso, H. 2015. Optimization of oil palm plant growth in Spodosol land. Jurnal Pertanian Tropik 2(3): 340- 347.

Tallgren, A.H., Airaksinen, U., von Weissenberg, R., Ojamo, H., Kuusisto, J. and Leisola, M. 1999. Exopolysaccharide-producing bacteria from sugar beets. Journal Applied Environmental Microbiology 65(2):862-864

Torres, C.A.V., Marques, R., Antunes, S., Alves, V.D., Sousa, I., Ramos, A.M., Oliveira, R., Freitas, F. and Reis, M.A.M., 2012. Study of the interactive effect of temperature and pH on exopolysaccharide production by Enterobacter A47 using multivariate statistical analysis. Journal of Biotechnology 119: 148–156

Yatno, E. 2011. Role of organic materials in improving soil physical quality and plants production. Jurnal Sumberdaya Lahan 5 (1):175-182