Glyphosate biodegradation by plant growth promoting bacteria and their effect to paddy germination in glyphosate contaminated soil

Lutfi Tri Andriani; Luqman Qurrata Aini; Tutung Hadiastono

doi:10.15243/jdmlm.2017.051.995

Glyphosate biodegradation by plant growth promoting bacteria and their effect to paddy germination in glyphosate contaminated soil

Lutfi Tri Andriani, Luqman Qurrata Aini, Tutung Hadiastono

J. Degrade. Min. Land Manage. , pp. 995-1000

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DOI https://doi.org/10.15243/jdmlm.2017.051.995

Abstract

Glyphosate is the most widely used herbicide in Indonesia. Glyphosate persistence between 55 days to 3 years. Widespread and uncontrolled use can cause weeds to become resistant and residue contaminates the soil and water environment. Due to the residual impact of glyphosate, it is necessary to identify a method that can increase the degradation of glyphosate. Several studies have shown that glyphosate can be degraded by microorganisms (fungi, rhizosphere and endophytic bacteria), some of which are members of plant growth-promoting bacteria. This study used the bacteria Enterobacter cloacae, Enterobacter sp and Pseudomonas fluorescens. These three types of bacteria have growth-promoting properties and potentially increase glyphosate degradation. Results of chromatogram on the residual test of glyphosate in liquid medium and soil containing glyphosate showed that glyphosate residue decreased with the addition of bacterial treatment when compared to control. The percentage of degradation in liquid medium are 96.06% by Enterobacter cloacae, 57% by Enterobacter spand 93.45% by Pseudomonas fluorescens.The percentage of degradation in soil medium are 4.32% by Enterobacter cloacae, 23.49% by Enterobacter spand 12.19% by Pseudomonas fluorescens.A positive result indicates that bacterial growth boosters from the plant (endophyte) as well as the area of rooting (rhizosphere) have additional potential as biofertilizer, bio stimulant, bio protectant but also as bio degradator pollutants such as the herbicide glyphosate

Keywords

bacteria; degradation; endophyte; glyphosate; rhizosphere

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References

Bashan, L.E., Hernandes, J.P., Bashan, Y. 2012. The potential contribution of plant growth – promoting bacteria to reduce environmental degradation – a comprehensive evaluation. Apllied Soil Ecology 61: 171-189

Bento, CP., Yang, X., Gort, G., Xue, S., Van Dam R., Zomer, P., Mol, H.G., Ritsema, C.J, and Geissen, V.2016. Persistence of glyphosate and aminomethylphosphonic acid in loess soil under different combinations of temperature, soil moisture and light/darkness. Science of the Total Environment 572 :301–311

Maharina, K.E. 2016. The Potential of Endophytic Bacteria in Increasing Growth and Physiological Activity of Potato Plant (Solanum tuberosum L.) Nadia Varieties under Drought Stress. Master Thesis, Brawijaya University, Malang (in Indonesian).

Kryuchkova, Y.V., Gennady L.B., Natalia E.G., Yuri V.G., Marina P.C., Oleg E.M., Evgenii E.F., and Olga V.T. 2014. Isolation and characterization of a glyphosate-degrading rhizosphere strain Enterobacter cloacae K7. Journal of Microbiological Research 169: 99-105

Pant, R., Pandey, P. and Kotoky, R. 2016. Rhizosphere mediated biodegradation of 1, 4-dichlorobenzene by plant growth promoting rhizobacteria of Jatrophacurcas. Ecological Engineering 94: p.50-56.

Pollegioni, L., Schonburn, E., Siehl, D. 2011. Molecular basis of glyphosate resistance: different approaches through protein engineering. FEBS Journal 278(16): 2753-2766

Sawhney, P.K., Kumar, A., Devi, U., Kumar, L., Kumar, N., Chhillar, A.K., Sharma, D.K., Bedi, J.S. and Saini,A.K. 2015. Isolation and anlysis of biodegradation potential of the bacterial endophytes Acinetobacter bejrenckii B5 and Enterobacter aerogens B8, against chlorpyrifos in vitro and in plantae. Octa Journal of Environmental Research 3(4): 317-326.

Schlegel, H.G. 1994. General Microbiology. Translated to Indonesian by Tedjo Baskoro. Gadjah Mada University Press. Yogyakarta

Travaglia, C., Masciarelli, O., Fortuna, J., Marchetti, G., Cardozo, P., Lucero, M., Zorza, E., Luna, V. and Reinoso, H. 2015. Towards sustainable maize production: glyphosate detoxification by Azospirillum sp. and Pseudomonas sp. Crop Protection 77: 102-109.

Yu, X.M., Yu, T., Yin, G.H., Dong, Q.L., An, M., Wang, H.R. and Ai, C.X. 2015. Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15. Genetics and Molecular Research 14 (4): 14717-14730.