Isolation of Indigenous Bacteria of Phosphate Solubilizing from Green Bean Rhizospheres

N Arfarita; M W Lestari; I Murwani; T Higuchi

doi:10.15243/jdmlm.2017.043.845

Isolation of Indigenous Bacteria of Phosphate Solubilizing from Green Bean Rhizospheres

N Arfarita, M W Lestari, I Murwani, T Higuchi

J. Degrade. Min. Land Manage. , pp. 845-851

Viewed : 1604 times

DOI https://doi.org/10.15243/jdmlm.2017.043.845

Abstract

The use of phosphate-solubilizing bacteria (PSB) as a biological fertilizer of Agricultural land is one solution to overcome problem of phosphate availability for plants. However, often application of a biological fertilizer is ineffective for certain places. The purpose of this study was to obtain indigenous phosphate solubilizing bacterial isolates that can be effective in the area of Malang. Samples were collected from rhizosphereof green bean plants at three locations in Malang, East Java. The study was conducted to determine the total bacterial population of soil samples, to select the best three bacterial isolates in phosphate solubilizing ability, which is not antagonistic and nonpathogenic for plants,along with observing its potential as a bacterial consortium. The highest total population was found in FHR samples of 1.5x10¹¹ CFU / mL. We have selected three bacterial isolates namely SPP1, SPP2 and SPP3. They were not antagonistic to each other and nonpathogenic on mungbean sprouts. They had possibility of producing growth hormone which characterized by an increasing in length of plant and total root length, be compared to controls. Strain SPP2 has shown the highest activity of phosphate solubilization then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SPP2 had 99% similarity with Pseudomonas plecoglossicida strain PR19

Keywords

consortium; indigenous bacteria; Malang; phosphate solubilizing

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References

Afarita, N., Djuhari, D., Prasetya, B. and Imai, T. 2016a. The application of Trichoderma viride strain FRP 3 for biodegradation of glyphosate herbicide in contaminated land. Agrivita Journal of Agricultural Science 38(3): 275-281.doi: http://doi.org/10.17503/agrivita.v38i3.550

Arfarita, N., Hidayati, N., Rosyidah, A., Machfudz, M. and Higuchi, T. 2016b. Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer. Journal of Degraded and Mining Lands Management 4(1):697-702. doi:10.15243/jdmlm.2016.041.697

Abril, A., Zurdo-Pineiro J.L., Peix, A., Rivas R. and Velazquez, E. 2007. Solubilization of phosphate by a strain of Rhizobium leguminosarum bv. Trifolii isolated from Phaseolus vulgaris in El Chaco Arido soil (Argentina) In: Velazquez E, Rodriguez-Berrueco C, editors. Developments in Plant and Soil Sciences. The Netherlands: Springer, 135–138.

Borneman, J. and Triplett, E.W. 2002. Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation. Applied Environmental Microbiology 63(7): 2647–2653.

Buckman, H. O. and Brady, N. C. 1982. Soil Science. Gajah Mada University Press, Yogyakarta, Indonesia.

Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, F.T., Lai, W.A., and Young, C.C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34:33–41. doi: 10.1016/j.apsoil.2005.12.002.

Chung, H., Park, M., Madhaiyan, M., Seshadri, S., Song, J. and Cho, H.S.T. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biology and Biochemitry 37:1970–1974. doi: 10.1016/j.soilbio.2005.02.025.

De Freitas, J.R., Banerjee, M.R. and Germida, J.J. 1997. Phosphate-solubilizing rhizobacteria enhance the growth and yield but not phosphorus uptake of canola (Brassica napus L.) Biology and Fertility of Soils 24:358–364. doi: 10.1007/s003740050258.

Kaur, G., and Reddy, M.S. 2014. Role of phosphate-solubilizing bacteria in improving the soil fertility and crop productivity in organic farming, Archives of Agronomy and Soil Science 60(4):549-564.

Handayanto, E., and Hairiah, K. 2007. Biologi Tanah: Landasan Pengelolaan Tanah Sehat. Pustaka Adipura, Yogyakarta, Indonesia.

Hilda, R. and Fraga, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnoly Advances 17:319–359.

Hasanudin and Bambang, G.M., 2004. Pemanfaatan mikrobia pelarut posfat dan mikoriza untuk perbaikan fospor tersedia, serapan fospor tanah (Ultisol) dan hasil jagung (pada Ultisol). Jurnal Ilmu-Ilmu Pertanian Indonesia 6(1):8-13.

Kumar, V., Behl, R.K. and Narula, N. 2001. Establishment of phosphate- solubilizing strains of Azotobacter chroococcum in the rhizosphere and their effect on wheat cultivars under greenhouse conditions. Microbiology Research 156:87–93. doi: 10.1078/0944-5013-00081.

McGill, W.B. and Cole, C.V. 1981. Comparative aspects of cycling of organic C, N, S and P through soil organic matter. Geoderma 26:267–268. doi: 10.1016/0016-7061(81)90024-0.

Nuraini, Y., Arfarita, N. and Siswanto, B. 2015. Isolation and characteristic of nitrogen-fixing bacteria and phosphate-solubilizing bacteria from soil high in mercury in tailings and compost areas of artisanal gold mine. Agrivita Journal of Agricultural Science 37(1): 1-7.

Sridevi, M., Mallaiah, K.V. and Yadav, N.C.S. 2007. Phosphate solubilization by Rhizobium isolates from Crotalaria species. Journal of Plant Science 2:635–639. doi: 10.3923/jps.2007.635.639.

Subba-Rao, N.S. 1995. Soil Microorganisms and Plant Growth. Science Pub Inc; 3 edition (December 1995), 350 pages

Suntoro. 2003. Peranan Bahan Organik terhadap Kesuburan Tanah dan Upaya Pengelolaan. Sebelas Maret University Press. Surakarta-Indonesia.

Torsvik, V. and Øvreås, L. 2002. Microbial diversity and function in soil: from genes to ecosystems. Current Opinion in Microbiology 5,240-245.

Vazquez, P., Holguin, G., Puente M, Lopez-cortes, A. and Bashan, Y. 2000. Phosphate solubilizing microorganisms associated with the rhizosphere of mangroves in a semi-arid coastal lagoon. Biology and Fertility of Soils. 30:460–468. doi: 10.1007/s003740050024.

Wani, P.A., Zaidi, A., Khan, A.A. and Khan, M.S. 2005. Effect of phorate on phosphate solubilization and indole acetic acid (IAA) releasing potentials of rhizospheric microorganisms. Annals Plant Protection Science 13:139–144.

Zaidi, A., Khan, M.S., Ahemad, M., Oves, M. and Wani, P.A. 2009. Recent Advances in Plant Growth Promotion by Phosphate-Solubilizing Microbes. In: Khan MS, et al., editors. Microbial Strategies for Crop Improvement. Berlin Heidelberg: Springer-Verlag 23–50.

Zhu, F., Qu, L., Hong, X. and Sun, X. 2011. Isolation and characterization of a phosphate-solubilizing halophilic bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the coast of Yellow Sea of China. Evid base Compl Alternative Med615032:six