Development of an indigenous bacterial consortium for enhanced oil degradation in saline-contaminated soils
DOI:
https://doi.org/10.15243/jdmlm.2025.124.7923Keywords:
biodegradation, enhanced oil degradation, indigenous bacteria, oil degradation, saline-contaminated soilsAbstract
This study developed the indigenous CR3.M3 bacterial consortium to enhance oil degradation in saline-contaminated soils. Seven hydrocarbon-degrading strains-closely related to Pseudomonas, Bacillus, and Niveispirillum species (92-99% 16S rRNA sequence similarity)-were isolated from polluted coastal soils using mineral salt media supplemented with crude oil and diesel. While phylogenetic analysis suggests close relationships to known oil-degrading species, formal taxonomic classification requires further genomic validation. The consortium degraded 70% of hydrocarbons within 13 days under saline conditions (?3% NaCl). Field trials in non-sterilized soils (3,542 mg/kg TPH) achieved 65.42% oil removal alongside microbial density increases from 6.26 to 8.11 Log??(CFU/g), confirming ecological compatibility. Its performance in both sterilized and native soils highlights adaptability for coastal bioremediation. Future research should optimize strain ratios, resolve taxonomic identities through whole-genome sequencing, and assess long-term ecological impacts to advance this sustainable remediation strategy.
References
Adams, G.O., Fufeyin, P.T., Okoro, S.E. and Ehinomen, I. 2015. Bioremediation, biostimulation and bioaugmention: a review. International Journal of Environmental Bioremediation & Biodegradation 3(1):28-39. https://doi.org/10.12691/ijebb-3-1-5
Al-Marri, S., Eldos, H., Ashfaq, M., Saeed, S., Skariah, S., Varghese, L., Mohamoud, Y., Sultan, A. and Raja, M. 2023. Isolation, identification, and screening of biosurfactant-producing and hydrocarbon-degrading bacteria from oil and gas industrial waste. Biotechnology Reports 39:e00804. https://doi.org/10.1016/j.btre.2023.e00804
Ayed, H.B., Jemil, N., Maalej, H., Bayoudh, A., Hmidet, N. and Nasri, M. 2015. Enhancement of solubilization and biodegradation of diesel oil by biosurfactant from Bacillus amyloliquefaciens An6. International Biodeterioration & Biodegradation 99:8-14. https://doi.org/10.1016/j.ibiod.2014.12.009
Boregowda, N., Jogigowda, S.C., Bhavya, G., Sunilkumar, C.R., Geetha, N., Udikeri, S.S., Chowdappa, S., Govarthanan, M. and Jogaiah, S. 2022. Recent advances in nanoremediation: Carving sustainable solution to clean-up polluted agriculture soils. Environmental Pollution 297:118728. https://doi.org/10.1016/j.envpol.2021.118728
Imperato, V., Portillo-Estrada, M., McAmmond, B.M., Douwen, Y., Van Hamme, J.D., Gawronski, S.W., Vangronsveld, J. and Thijs, S. 2019. Genomic diversity of two hydrocarbon-degrading and plant growth-promoting Pseudomonas species isolated from the oil field of Bóbrka (Poland). Genes (Basel) 10(6). https://doi.org/10.3390/genes10060443
Kebede, G., Tafese, T., Abda, E.M., Kamaraj, M. and Assefa, F. 2021. Factors influencing the bacterial bioremediation of hydrocarbon contaminants in the soil: mechanisms and impacts. Journal of Chemistry 2021(1), 15 November 2021:9823362. https://doi.org/10.1155/2021/9823362
Kumar, G. and Reddy, K.R. 2020. Addressing climate change impacts and resiliency in contaminated site remediation. Journal of Hazardous, Toxic, and Radioactive Waste 24(4):04020026. https://doi.org/10.1061/(ASCE)HZ.2153-5515.0000515
Kupryashina, M.A., Petrov, S.V., Ponomareva, E.G. and Nikitina, V.E. 2015. Ligninolytic activity of bacteria of the genera Azospirillum and Niveispirillum. Microbiology 84(6):691-691. https://doi.org/10.7868/S0026365615060051
Nwaogu, L., Onyeze, G. and Nwabueze, R. 2008. Degradation of diesel oil in a polluted soil using Bacillus subtilis. African Journal of Biotechnology 7(12):1939. https://doi.org/10.5897/AJB07.889
Ozyurek, S.B and Bilkay, I.S. 2020. Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit. SN Applied Sciences 2:1-12. https://doi.org/10.1007/s42452-020-2044-5
Parthipan, P., Preetham, E., Machuca, L.L., Rahman, P.K., Murugan, K. and Rajasekar, A. 2017. Biosurfactant and degradative enzymes mediated crude oil degradation by bacterium Bacillus subtilis A1. Frontiers in Microbiology 193. https://doi.org/10.3389/fmicb.2017.00193
Rodrigues, E.M., Kalks, K.H. and Totola, M.R. 2015. Prospect, isolation, and characterization of microorganisms for potential use in cases of oil bioremediation along the coast of Trindade Island, Brazil. Journal of Environmental Management 156:15-22. https://doi.org/10.1016/j.jenvman.2015.03.016
Sakthipriya, N., Doble, M. and Sangwai, J.S. 2015. Bioremediation of coastal and marine pollution due to crude oil using a microorganism Bacillus subtilis. Procedia Engineering 116:213-220. https://doi.org/10.1016/j.proeng.2015.08.284
Satpute, S.K., Banpurkar, A.G., Dhakephalkar, P.K., Banat, I.M. and Chopade, B.A. 2010. Methods for investigating biosurfactants and bioemulsifiers: a review. Critical Reviews in Biotechnology 30(2):127-144. https://doi.org/10.3109/07388550903427280
Shah, G. and Soni, V. 2024. Comprehensive insights into the impact of oil pollution on the environment. Regional Studies in Marine Science 734:103516. https://doi.org/10.1016/j.rsma.2024.103516
Sudarmono, P., Wibisana, A., Listriyani, L.W. and Sungkar, S. 2019. Characterization and synergistic antimicrobial evaluation of lipopeptides from Bacillus amyloliquefaciens isolated from oil?contaminated soil. International Journal of Microbiology 2019(1):3704198. https://doi.org/10.1155/2019/3704198
Tuhuloula, A., Suprapto, S., Altway, A. and Juliastuti, S.R. 2019. Biodegradation of extractable petroleum hydrocarbons by consortia Bacillus cereus and Pseudomonas putida in petroleum contaminated-soil. Indonesian Journal of Chemistry 19(2):347-355. https://doi.org/10.22146/ijc.33765
Tuyen, D.T., Thanh, N.T.K., Khoa, N.X.B. and Cuong, N.C. 2022. Identification of hydrocarbon-degrading bacterial consortium isolated from the oil-contaminated muddy soil in Hanoi, Vietnam. Povolzhskiy Journal of Ecology 2:206-215. https://doi.org/10.35885/1684-7318-2021-3-348-357
Usman, Z., Said, M., Shehu, F., Abdussalam, K., Abdulrazak, T. and Abdullahi, N. 2021. Isolation and identification of phenol-degrading bacteria from oil-contaminated sites. Nigerian Journal of Biotechnology 38(1):160-165. https://doi.org/10.4314/njb.v38i1.19
Xu, Y., Teng, Y., Wang, X., Ren, W., Zhao, L., Luo, Y., Christie, P. and Greening, C. 2023. Endogenous biohydrogen from a rhizobium-legume association drives microbial biodegradation of polychlorinated biphenyl in contaminated soil. Environment International 176. https://doi.org/10.1016/j.envint.2023.107962
Zhang, Z., Gai, L., Hou, Z., Yang, C., Ma, C., Wang, Z., Sun, B., He, X., Tang, H. and Xu, P. 2010. Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils. Bioresource Technology 101(21):8452-8456. https://doi.org/10.1016/j.biortech.2010.05.060
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