Role of plant growth promoting rhizobacteria on Pteris vittata L as a potential hyperaccumulator plant for gold phytomining agent
DOI:
https://doi.org/10.15243/jdmlm.2025.122.7217Keywords:
gold, phytomining, Pteris vittata L, Ratatotok, rhizobacteriaAbstract
Gold phytomining is the extraction of gold from the soil by harvesting specially selected hyperaccumulator plants. One of the potential plant species as a gold hyperaccumulator at the Ratatotok site, North Sulawesi, Indonesia, is Pteris vitata L, possibly because of the presence of rhizobacteria colonies in the roots to help the plant's resistance to metal stress in the soil. The isolation and identification show that the most resistant rhizobacteria to the gold stress is Pseudomonas aeruginosa RTKP1. The study aimed to assess the Pseudomonas aeruginosa RTKP1 to assist the gold bioaccumulation in Pteris vittata L. The phytomining test was carried out with four series of treatments for Pteris vittata L growing media, i.e., (1) tailings without compost and bacterial isolates, (2) tailings with bacterial isolates, (3) tailings with compost, and (4) tailings with compost and bacterial isolate. A descriptive analysis was carried out to analyze the role of the Pseudomnas aeruginosa RTKP1 on gold reduction in the media and gold bioaccumulation in Pteris vittata L. A quantitative analysis was carried out to analyze the bioaccumulation ability of Pteris vittata L with and without the addition of bacterial isolate. The role of the Pseudomonas aeruginosa RTKP1 is to increase the Translocation Factor (TF) and Biological Concentration Factor (BCF), particularly in the roots. The addition of compost to tailings as growing media for Pteris vittata L inhibits the effectiveness of the work of the Pseudomonas aeruginosa RTKP1 in increasing BCF and TF. However, this effect needs to be tested further to obtain significant results.
References
Abtahi, H., Parhamfar, M., Saeedi, R., Villaseñore, J., Sartajf, M., Vinod, K., Coulon, F., Parhamfar, M., Didehdar, M., Seifi, H. and Koolivand, A. 2020. Effect of competition between petroleum-degrading bacteria and indigenous compost microorganisms on the efficiency of petroleum sludge bioremediation: Field application of mineral-based culture in the composting process, Journal of Environmental Management 258:110013. https://doi.org/10.1016/j.jenvman.2019.110013
Aminatun, T., Idrus, A., Simbolon, D., Rakhmawati, A. and Atun, S. 2024a. Lithogeochemical characteristics and potential hyperaccumulator identification as phytomining agent at the Ratatotok gold mine, Indonesia. Journal of Degraded and Mining Lands Management 11(2):5251-5261. https://doi.org/10.15243/jdmlm.2024.112.5251
Aminatun, T., Rakhmawati, A., Atun, S., Idrus, A., Simbolon, D.R. and Restele, L 2024b. Characteristics of rhizobacteria in potential hyperaccumulator vegetation and their resistance to gold mine tailing stress. Journal of Water and Land Development 1(60):209-218. https://doi.org/10.24425/jwld.2024.149122
Azzaman, M.A., Idrus, A. and Titisari, A.D. 2021. Geology, hydrothermal alteration and mineralization of the Carlin-type gold deposit at South Ratatotok, Southeast Minahasa Regency, North Sulawesi Province, Indonesia. IOP Conference Series: Earth and Environmental Science 789(1). https://doi.org/10.1088/1755-1315/789/1/012076
Benidire, L., Madline, A., Pereira, S.I.A., Castro, P.M.L. and Boularbah, A. 2021. Synergistic effect of organo-mineral amendments and plant growth-promoting rhizobacteria (PGPR) on the establishment of vegetation cover and amelioration of mine tailings. Chemosphere 262:127803. https://doi.org/10.1016/j.chemosphere.2020.127803
Betancur-Corredor, B., Loaiza-Usuga, J.C., Denich, M. and Borgemeister, C. 2018. Gold mining as a potential driver of development in Colombia: Challenges and opportunities. Journal of Cleaner Production 199:538-553. https://doi.org/10.1016/j.jclepro.2018.07.142
Chtouki, M., Naciri, R., Soulaimani, A., Zeroual, Y., El Gharous, M. and Oukarroum, A. 2021. Effect of cadmium and phosphorus interaction on tomato: Chlorophyll a fluorescence, plant growth, and cadmium translocation. Water, Air, and Soil Pollution 232(3). https://doi.org/10.1007/s11270-021-05038-x
Dinh, T., Dobo, Z. and Kovacs, H. 2022. Phytomining of noble metals - A review. Chemosphere 286:131805. https://doi.org/10.1016/j.chemosphere.2021.131805
Funari, R., Ripa, R., Soderstrom, B., Skoglund, U. and Shen, A.Q. 2019. Detecting gold biomineralization by Delftia acidovorans biofilms on a quartz crystal microbalance. ACS Sensors 4(11):3023-3033. https://doi.org/10.1021/acssensors.9b01580
Gagnon, V., Rodrigue-Morin, M., Migneault, M., Tardif, A., Garneau, L., Lalonde, S., Shipley, B., Greer, C.W., Bellenger, J.P. and Roy, S. 2020. Survival, growth and element translocation by 4 plant species growing on acidogenic gold mine tailings in Québec. Ecological Engineering 151:105855. https://doi.org/10.1016/j.ecoleng.2020.105855
Govarthanan, M., Mythili, R., Selvankumar, T., Kamala-Kannan, S., Rajasekar, A. and Chang, Y.C. 2016. Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. RM isolated from the roots of Tridax procumbens. 3 Biotech 6(242):2-7. https://doi.org/10.1007/s13205-016-0560-1
Herlina, L., Widianarko, B., Purnaweni, H., Sudarno, S. and Sunoko, H.R. 2020. Phytoremediation of lead contaminated soil using croton (Cordiaeum variegatum) plants. Journal of Ecological Engineering 21(5):107-113. https://doi.org/10.12911/22998993/122238
Jalali, J. and Lebeau, T. 2021. The role of microorganisms in mobilization and phytoextraction of rare earth elements: A review. Frontiers in Environmental Science 9(June):1-19. https://doi.org/10.3389/fenvs.2021.688430
Kohda, Y.H.T., Qian, Z., Chien, M.F., Miyauchi, K., Endo, G., Suzui, N., Yin, Y.G., Kawachi, N., Ikeda, H., Watabe, H., Kikunaga, H., Kitajima, N. and Inoue, C. 2021. New evidence of arsenic translocation and accumulation in Pteris vittata from real-time imaging using positron-emitting 74As tracer. Scientific Reports 11:12149. https://doi.org/10.1038/s41598-021-91374-1
Kulkarni, M.G., Stirk, W.A., Southway, C., Papenfus, H.B., Swart, P.A., Lux, A., Vaculík, M., Martinka, M. and van Staden, J. 2013. Plant growth regulators enhance gold uptake in Brassica Juncea. International Journal of Phytoremediation 15(2):117-126. https://doi.org/10.1080/15226514.2012.683207
Kurniawan, R., Hamim, Henny, C. and Satya, A. 2022. Identification of potential phytoaccumulator plants from tailings area as a gold phytomining agent. Journal of Ecological Engineering 23(1):169-181. https://doi.org/10.12911/22998993/143978
Li, J., Chang, Y., AL-Huqail, A.A., Ding, Z., Al-Harbi, M.S., Ali, E.F., Amany, H.A., Abeed, A.H.A., Rekaby, S.A., Eissa, M.A., Ghoneim, A.M. and Tammam, S.A. 2021. Effect of manure and compost on the phytostabilization potential of heavy metals by the halophytic plant wavy-leaved saltbush. Plants 10(10):1-13. https://doi.org/10.3390/plants10102176
Li, M., Cheng, X. and Guo, H. 2013. Heavy metal removal by biomineralization of urease producing bacteria isolated from soil. International Biodeterioration and Biodegradation 76:81-85. https://doi.org/10.1016/j.ibiod.2012.06.016
Maiti, S.B., Ghosh, D. and Raj, G. 2021. Phytoremediation of fly ash: Bioaccumulation and translocation of metals in natural colonizing vegetation on fly ash lagoons. Handbook of Fly Ash. Elsevier. pp. 501-523. https://doi.org/10.1016/B978-0-12-817686-3.00011-6
Mang, K.C. and Ntushelo, K. 2019. Phytoextraction and phytostabilisation approaches of heavy metal remediation in acid mine drainage with case studies: A review. Applied Ecology and Environmental Research 17(3):6129-6149. https://doi.org/10.15666/aeer/1703_61296149
Mesa-Marín, J., Del-Saz, N.F., Rodríguez-Llorente, I.D., Redondo-Gomez, S., Pajuelo, E., Ribas-Carbo, M. and Mateos-Naranjo, E. 2018. PGPR reduce root respiration and oxidative stress enhancing spartina maritima root growth and heavy metal rhizoaccumulation. Frontiers in Plant Science 2018 Oct 17:9:1500. https://doi.org/10.3389/fpls.2018.01500
Phyo, A.K., Jia, Y., Tan, Q., Sun, H., Liu, Y., Dong, B. and Ruan, R. 2020. Competitive growth of sulfate-reducing bacteria with bioleaching acidophiles for bioremediation of heap bioleaching residue. International Journal of Environmental Research and Public Health 17(8):1-14. https://doi.org/10.3390/ijerph17082715
Pramono, A., Rosariastuti, M.M.A.R., Ngadiman, and Prijambada, I.D. 2012. The role of rhizobacteria in phytoextraction of heavy metals. Jurnal Ecolab 6(1):38-50 (in Indonesian). https://doi.org/10.20886/jklh.2012.6.1.38-50
Reith, F., Lengke, M.F., Falconer, D., Craw, D. and Southam, G. 2007. The geomicrobiology of gold. ISME Journal 1(7):567-584. https://doi.org/10.1038/ismej.2007.75
Rodriguez, N., Carusso, S., Juarez, A., Kassisse, Y.E., Salemi, V.R. and de Cabo, L. 2023. Effect of stabilization time and soil chromium concentration on Sesbania virgata growth and metal tolerance. Journal of Environmental Management 345:118701. https://doi.org/10.1016/j.jenvman.2023.118701
Sheoran, V., Sheoran, A.S. and Poonia, P. 2013. Phytomining of gold: A review. Journal of Geochemical Exploration 128:42-50. https://doi.org/10.1016/j.gexplo.2013.01.008
USGS. 2022. National Minerals Information Center, Gold Statistics and Information. Statistics and information on the worldwide supply of, demand for, and flow of the mineral commodity gold. https://www.usgs.gov/centers/national-minerals-information-center/gold-statistics-and-information.
Wang, X., Jiang, H., Zheng, G., Liang, J. and Zhou, L. 2021 Recovering iron and sulfate in the form of mineral from acid mine drainage by a bacteria-driven cyclic biomineralization system. Chemosphere 262:127567. https://doi.org/10.1016/j.chemosphere.2020.127567
Yaylali-Abanuz, G., Tüysüz, N. and Akaryali, E. 2012. Soil geochemical prospection for gold deposit in the Arzular area (NE Turkey). Journal of Geochemical Exploration 112:107-117. https://doi.org/10.1016/j.gexplo.2011.08.004
Yoon, J., Cao, X., Zhou, Q. and Ma, L.Q. 2006. Accumulation of Pb, Cu, and Zn in native plants growing on a contaminated Florida site. Science of the Total Environment 368(2-3):456-464. https://doi.org/10.1016/j.scitotenv.2006.01.016
Zhou, Q., Li, R., Li, T., Zhou, R., Hou, Z. and Zhang, X. 2023. Interactions among microorganisms functionally active for electron transfer and pollutant degradation in natural environments. Eco-Environment and Health 2(1):3-15. https://doi.org/10.1016/j.eehl.2023.01.002
Downloads
Submitted
Accepted
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Journal of Degraded and Mining Lands Management

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Submission of a manuscript implies: that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis) that it is not under consideration for publication elsewhere; that if and when the manuscript is accepted for publication, the authors agree to automatic transfer of the copyright to the publisher.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Scientific Journal by Eko Handayanto is licensed under a Creative Commons Attribution 4.0 International License.
Based on a work at https://ub.ac.id.
Permissions beyond the scope of this license may be available at https://ircmedmind.ub.ac.id/.