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A Atekan
Pasca FPUB
Indonesia

Researcher

Y Nuraini
Brawijaya Univ
Indonesia

Senior Lecturer

E Handayanto
Brawijaya Univ
Indonesia

Professor

S Syekhfani
Brawijaya Univ
Indonesia

Professor

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The potential of phosphate solubilizing bacteria isolated from sugarcane wastes for solubilizing phosphate

A Atekan, Y Nuraini, E Handayanto, S Syekhfani
  J. Degrade. Min. Land Manage. , pp. 175-182  
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Abstract


Most of P in agricultural soils is in unavailable forms for plant growth. Phosphate solubilizing bacteria can increase soil P availability. This study was aimed to isolate phosphate solubilizing bacteria from sugarcane waste compost and to test ability of the isolated bacterial to dissolve phosphate. The bacteria were isolated from three types of sugarcane waste, i.e. filter cake compost, bagasse compost, and a mixture of filter cake + bagasse + trash biomass compost. The potential colony was further purified by the Pikovskaya method on selective media. Eight isolates of phosphate solubilizing bacteria were obtained from all wasted studied. Amongst them, T-K5 and T-K6 isolates were superior in dissolving P from Ca3(PO4)2 in the media studied. The two isolates were able to solubilize P with solubilizing index of 1.75 and 1.67 for T-K5 and T-K6, respectively. Quantitatively, T-K6 isolate showed the highest P solubilization (0.74 mg / L), followed by T-K5 isolate (0.56 mg / L), while the lowest P solubilization (0.41 mg / L) was observed for T-K4 isolate. The increase of soluble P was not always followed by the decrease in pH.


Keywords


organic waste; phosphorus; phosphate solubilizing bacteria

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References


Arcand, M.M. and Schneider, K.D. 2006. Plant-and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: a review. Anais da Academia Brasileira de Ciências 78(4): 791–807.

Bashan, Y., Kamnev, A.A. and de-Bashan, L.E. 2013. Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure. Biology and Fertility of Soils 49: 465–479.

Behera, B.C., Singdevsachan, S.K., Mishra, R.R., Dutta, S.K. and Thatoi, H.N. 2014. Diversity, mechanism and biotechnology of phosphate solubilizing microorganism in mangrove: A review. Biocatalysis and Agricultural Biotechnology 3(2): 97–110.

Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, F.T., Lai, W.A. and Young, C.C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Applied Soil Ecology 34: 33–41.

Chung, H., Park, M., Madhaiyan, M., Seshadri, S., Song, J., Cho, H. and Sa, T. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biology and Biochemistry 37: 1970–1974.

Goldstein, A.H.1995. Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilisation by Gram-negative bacteria. Biological Agriculture and Horticulture 12: 185–193.

Gupta, M., Kiran, S., Gulati, A., Singh, B. and Tewari, R. 2012. Isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-A biosynthesis of Aloe barbadensis Miller. Microbiological Research 167: 358– 363.

Gyaneshwar, P., Kumar, N.J., Pareka, L.J. and Podle, P.S. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant and Soil 245(1):83–93.

Hameeda, B., Harini, G., Rupela, O.P., Wani, S.P. and Reddy, G. 2008. Growth promotion of maize by phosphate solubilizing bacteria isolated from composts and macrofauna. Microbiological Research 163: 234—242

Holford, I.C.R.1997. Soil phosphorus its measurement and its uptake by plants. Australian Journal of Soil Research 35: 227–239.

Holguin, G., Vazquez, P. and Bashan, Y. 2001. The role of sediment microorganisms in the productivity, conservation and rehabilitation of mangrove ecosystems: an overview. Biology and Fertility of Soils 33: 265–278.

Khan, A.A., Jilani, G., Akhtar, M.S., Naqvi, S.M.S. and Rasheed, M. 2009. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production. Journal of Agriculture and Biological Science 1(1): 48-58.

Khan, M.S., Zaidi, A. and Wani, P.A. 2007. Role of phosphate-solubilizing microorganisms in sustainable agriculture – A review. Agronomy and Sustainable Development 27: 29-43.

Kim, K.Y., Jordan, D. and McDonald, G.A.1997. Solubilization of hydroxyl apatite by Enterobacter agglomerans and cloned Escherichiacoli in culture medium. Biology and Fertility of Soils 24: 347–352.

Kpomblekou-A, K. and Tabatabai, M.A. 2003. Effect of low-molecular weight organic acids on phosphorus release and phytoavailabilty of phosphorus in phosphate rocks added to soils. Agriculture, Ecosystems and Environment 100: 275–284.

Kpomblekou-A, K. and Tabatabai, M.A. 1994. Effect of organic acids on release of phosphorus from phosphate rocks. Soil Science 158: 442–453.

Nuraini, Y., Abadi, A.L., Soemarno. and Ardyati, T. 2011. Potensial of legume and maize to stimulate population of nitrogen-fixing bacteria, phosphate-solubilizing bacteria and indole acetic acid production. Journal of Agriculte and Food Technology 1(12): 218-226.

Oliveira, C.A., Alves, V.M.C., Marriel, I.E., Gomes, E.A., Scotti, M.R., Carneiro, N.P., Guimara˜es, C.T., Schaffert, R.E. and Sa, N.M.H. 2009. Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome. Soil Biology and Biochemistry 41: 1782–1787.

Poonguzhali, S., Madhaiyan, M. and Sa, T. 2008. Isolation and identification of phosphate solubilizing bacteria from chinese cabbage and their effect on growth and phosphorus utilization of plants. Journal of Microbiology and Biotechnology 18(4): 773-777.

Premono, M.E., Moawad, A.M. and Vleck, P.L.G. 1996. Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Science 11:13–23

Prochnow, L. I., Fernando, J., Quispe, S., Artur, E., Francisco, B. and Braga, G. 2006. Effectiveness of phosphate fertilizers of different water solubilities in relation to soil phosphorus adsorption, 1(August), 333–340.

Rao, N.S.S. 1982. Phosphate solubilization by soil microorganisms. In N.S. Rao (ed.) Advanced in Agricultural Microbiology. New Delhi: Oxford and IBH Publishing Co.

Rashid, M., Khalil, S., Ayub, N., Alam, S. and Latif, F. 2004. Organic acids production and phosphate solubilization by phosphate solubilization microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Sciences 7(2): 187-196.

Stevenson, F.J. 1986. Cycles of Soil Carbon, Nitrogen, Phosphorus, Sulphur and Micronutrients. Wiley, New York.

Tao, G.C., Tian S.J., Cai, M.Y. and Xie, G.H. 2008. Phosphate-solubilizing and mineralizing abilities of bacteria isolated from soils. Pedosphere 18(4): 515–523.

Vazquez, P., Holguin, G., Puente, M.E., Lopez-Cortes, A. and Bashan, Y. 2000. Phosphate-solubilizing microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biology and Fertility of Soils 30 (5-6): 460-468.

Watanabe, F.S. and Olsen, S.R. 1965. Test of an ascorbic acid method for determining phosphorous in water and NaHCO3 extracts from soil. Soil Science Society of America

Widawati, S. and Suliasih. 2006. Augmentation of potential phosphate solubilizing bacteria (PSB) stimulate growth of green mustard (Brasica caventis Oed.) in marginal soil. Biodiversitas 7(1): 10-14.

Widawati, S., Nurkanto, A. and Sudiana, I.M. 2008. Phosphate solubilizing activities of Actinomycetes isolated from Waigeo, Raja Ampat islands, West Papua. Biodiversitas 9(2): 87-90.

Yang, M., Ding, G., Shi, L., Xu, F. and Meng, J. 2010. Detection of QTL for phosphorus efficiency at vegetative stage in Brassica napus. Plant and Soil 339 (1-2): 97–111. doi:10.1007/s11104-010-0516-x.

Yu, X., Liu, X., Zhu, T.H., Liu, G.H. and Mao, C. 2011. Isolation and characterization of phosphate-solubilizing bacteria from walnut and their effect on growth and phosphorus mobilization. Biology and Fertility of Soils 47: 437–446.


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